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DETECCION MOLECULAR DE ENTEROCOCCUS FAECALIS EN CONDUCTOS RADICULARES CON FRACASO ENDODONTICO

MARCO ANTONIO RAMIREZ SALOMON (2010)

The treatment of root canals presents an index of failure, according to international publications, between 5 and 10%. In a large proportion of these (from 24 to 77%), bacteria of the species Enterococcus faecalis have been identified. The objective of this study was to determine the presence of Enterococcus faecalis in root canals with unsuccessful root treatment in patients from the city of Mérida, Yucatán, Mexico.

El tratamiento de conductos radiculares presenta un índice de fracaso, según publicaciones internacionales, entre el 5 y 10%. En gran proporción de éstos (del 24 al 77%), se han identificado bacterias de la especie Enterococcus faecalis. El objetivo de este estudio fue determinar la presencia de Enterococcus faecalis en conductos de dientes con tratamiento radicular fracasado efectuado en pacientes de la ciudad de Mérida, Yucatán, México.

Master thesis

Molecular Fracaso endodóntico Conductos radiculares Enterococcus faecalis   Endodontic failure   Root canals MEDICINA Y CIENCIAS DE LA SALUD

Comparison of Enterococcus faecium and Enterococcus faecalis Strains Isolated from Water and Clinical Samples: Antimicrobial Susceptibility and Genetic Relationships.

Gonzalo Castillo Rojas Marisa Mazari Hiriart Sergio Ponce de León Rosales Rosa Amieva Raul Azael Agis Juarez Johannes Huebner Yolanda Lopez Vidal (2013)

Enterococci are part of the normal intestinal flora in a large number of mammals, and these microbes are currently used as indicators of fecal contamination in water and food for human consumption. These organisms are considered one of the primary causes of nosocomial and environmental infections due to their ability to survive in the environment and to their intrinsic resistance to antimicrobials. The aims of this study were to determine the biochemical patterns and antimicrobial susceptibilities of Enterococcus faecalis and E. faecium isolates from clinical samples and from water (groundwater, water from the Xochimilco wetland, and treated water from the Mexico City Metropolitan Area) and to determine the genetic relationships among these isolates. A total of 121 enterococcus strains were studied; 31 and 90 strains were isolated from clinical samples and water (groundwater, water from the Xochimilco wetland, and water for agricultural irrigation), respectively. Identification to the species level was performed using a multiplex PCR assay, and antimicrobial profiles were obtained using a commercial kit. Twenty-eight strains were analyzed by pulsed-field gel electrophoresis (PFGE). E. faecium strains isolated from water showed an atypical biochemical pattern. The clinical isolates showed higher resistance to antibiotics than those from water. Both the enterococci isolated from humans, and those isolated from water showed high genetic diversity according to the PFGE analysis, although some strains seemed to be closely related. In conclusion, enterococci isolated from humans and water are genetically different. However, water represents a potential route of transmission to the community and a source of antimicrobial resistance genes that may be readily transmitted to other, different bacterial species.

Article

Bacteriología Enterococcus faecalis Resistencia antimicrobiana MEDICINA Y CIENCIAS DE LA SALUD

Aislamiento y evaluación in vitro de dos bacterias acetogénicas sobre la producción de metano (CH4) en cocultivos con bacterias ruminales.

IVAN REYES VAZQUEZ (2015)

Tesis (Doctorado en Ciencias, especialista en Ganadería).- Colegio de Postgraduados, 2015.

Mediante un medio de cultivo anaerobio a base de acetato-formato fluido ruminal (AF-FR) se aislaron y conservaron en liofilización dos bacterias ruminales. La primer bacteria ruminal aislada se identificó molecularmente como Enterococcus casseliflavus (EC) a una concentración de 6.75 x 108 bacterias mL-1; la segunda bacteria solo se obtuvo el género Enterococcus (EN), a una concentración de 3.4x107 bacterias mL-1. Se comparó la eficiencia de ambas bacterias en medios de cultivo anaerobios a base de formato fluido ruminal (F-FR); glucosa, celobiosa y almidón fluido ruminal (GCA-FR) y el pasto Cynodon dactylon fluido ruminal (B-FR). Los datos se analizaron en un diseño completamente al azar con un arreglo factorial de tratamientos 2x3x3, y las medias se compararon con la prueba de Tukey. La interacción medio-inóculo, logró disminuir el CH4 en 35.51% en medios con F-FR en COC, siendo diferente estadísticamente (P≤0.05) de inóculo con la batería sola y FR. En los medios con GCA-FR no disminuyó la cantidad de CH4, mientras que los medios con B-FR se disminuyó solo el 9.02%. EC produjo mayor cantidad de acetato sin presentar diferencias significativas (P≤0.05) comparado con EN. No hay estudios que comprueben que bacterias del género Enterococcus disminuyan la producción de CH4. La bacteria EC es mejor que EN disminuyendo CH4 y produciendo mayor acetato. _______________ ISOLATION AND EVALUATION IN VITRO OF TWO ACETOGENIC BACTERIA ON PRODUCTION METHANE (CH4) IN COCULTURES WITH RUMINAL BACTERIA. ABSTRACT: Through an anaerobic culture medium based format acetate rumen fluid (AF-FR) they were isolated and conserved in two rumen bacteria lyophilization. The first ruminal bacterium isolated molecularly identified as Enterococcus casseliflavus (EC) at a concentration of 6.75 x 108 bacteria mL-1; the second bacterium only was obtained genus Enterococcus (EN) at a concentration of 3.4x107 bacteria mL-1. The efficiency of both bacteria in culture media anaerobic rumen fluid based format (F-FR) compared; glucose, cellobiose and starch ruminal fluid (GCA-FR) and the grass Cynodon dactylon rumen fluid (B-FR). The data were analyzed in a completely randomized design with a factorial arrangement of treatments 2x3x3, and the means were compared with Tukey's test. The medium-inoculum interaction able to reduce CH4 with 35.51% on average in F-FR COC, being statistically different (P≤0.05) inoculum on battery power alone and FR. Media with GCA-FR did not decrease the amount of CH4, while the means B-FR only 9.02% was decreased. EC acetate produced more without significant differences (P≤0.05) compared with EN. There are no studies that prove bacteria of the genus Enterococcus that reduce the production of CH4. The bacteria EC is better than EN to decreasing CH4 and producing more acetate.

Doctoral thesis

Aislamiento Enterococcus Metano Acetato Isolation Methane Acetate Ganadería Doctorado CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS CIENCIAS VETERINARIAS FISIOLOGÍA ANIMAL