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Propagación sexual y tolerancia a la desecación en nanche (Byrsonima crassifolia L) Kunth

CAMELIA JAIMES ALBITER (2013)

Tesis (Doctora en Ciencias, especialista en Producción de Semillas).- Colegio de Postgraduados, 2013.

El nanche es una especie forestal frutícola de amplio espectro utilitario, pero la germinación de sus semillas es compleja; por ello, el objetivo de la investigación fue conocer la dinámica germinativa de las semillas de clima cálido húmedo y clima semicálico, en el banco del suelo y en condiciones de invernadero; así como clasificarlas fisiológicamente, y proponer alternativas adecuadas para conservar este recurso fitogenético. Se encontró que las semillas de clima cálido húmedo forman un banco de tipo transitorio, en tanto que, para las de clima semicálido fue persistente de corto plazo. Por otro lado, en invernadero se observó que la humedad del suelo no representa inconveniente para la germinación de las semillas de ambos climas, si no que esto depende del grado de domesticación, la latencia mecánica y fisiológica, calidad física, fisiológica y sanitaria de las semillas. Respecto a la clasificación fisiológica de las semillas, se observó que las de clima semicálido son más tolerantes a la desecación que las de clima cálido húmedo; sin embargo, ninguna conserva su viabilidad durante el almacenamiento, por lo que se concluye que ambas son de tipo recalcitrante, y la mejor estrategia para conservar el nanche es in situ o ex situ: en colecciones de campo, jardines botánicos, cultivo de tejidos o mediante crioconservación. _______________ SEXUAL PROPAGATION AND DRYING TOLERANCE IN nanche (Byrsonima crassifolia L.) Kunth. ABSTRACT: The nanche fruit is a forest of broad utility, but seed germination is complex, hence, the aim of the research was to understand the dynamics of seed germination warm humid and semiwarm climate, respectively, in the soil bank, and greenhouse conditions as well as physiologically classify, and propose adequate alternatives of this plant genetic resource conservation. It was found that warm and humid climate, seeds form a transitional bank, while for the semiwarm climate is of persistent short term type. On the other hand, in the greenhouse it was observed that the soil moisture is not inconvenient for to seed germination in both climates, but rather it depends on the the degree of domestication, mechanical and physiological dormancy, physical quality, physiological and sanitary quality of seeds. About the physiological classification of seeds, it was observed that those from semiwarm climate are more desiccation tolerant than those from warm humid climate, however, its viability its not retained during storage, so it was concluded that both are of recalcitrant type, and the best strategy to conserve nanche is in situ or ex situ: in field collections, botanical gardens, tissue culture or by cryopreservation.

Doctoral thesis

Byrsonima crassifolia Latencia Recalcitrante Conservación in situ Conservación ex situ Dormancy In situ conservation Ex situ conservation Producción de Semillas Doctorado Recalcitrant CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Cloning and molecular characterization of a putative habanero pepper SERK1 cDNA expressed during somatic and zygotic embryogenesis

Doribet Jiménez Guillen DANIEL PEREZ PASCUAL RAMON ARMANDO SOUZA PERERA José Juan Zúñiga Aguilar (2019)

Plant gene homologs that control cell differentiation can be used as biotechnological tools to study the in vitro cell proliferation competence of tissue culture-recalcitrant species such as peppers. It has been demonstrated that SERK1 homologs enhance embryogenic competence when overexpressed in transformed tissues; therefore, cloning of a pepper SERK1 homolog was performed to further evaluate its biotechnological potential.

Article

ARABIDOPSIS BIOTECHNOLOGICAL TOOLS CAPSICUM ANNUUM CAPSICUM CHINENSE CELL DIFFERENTIATION GENE EXPRESSION IN VITRO CELL PROLIFERATION PEPPERS RECALCITRANT SPECIES BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR DE PLANTAS BIOLOGÍA MOLECULAR DE PLANTAS

Agrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris) var. Brunca

LAURA YESENIA SOLÍS RAMOS Julio César Ortiz Pavón ANTONIO ANDRADE TORRES Romano Porras Murillo Arturo Brenes Angulo Enrique Castaño de la Serna (2019)

Common bean is a crop recalcitrant to in vitro regeneration and therefore it lacks an efficient transformation protocol that can be reproduced using A. tumefaciens. The main goal of this study was to establish a protocol for A. tumefaciens mediated transformation of Phaseolus vulgaris var. Brunca by marker genes (gusA and nptII) together with the gene for trehalose-6-phosphate synthase from Saccharomyces cerevisiae (TPS1) used in other species to increase tolerance to abiotic stress. The β-glucuronidase activity was detected in 45 % of the LBA4404 ElectroMAX® pCAMBIA1301 infected explants. Transformed explants regenerated new shoots after four to five months period in a kanamycin rich media. Surviving plants were evaluated by PCR and presented an 0.5 % efficiency of transformation. The established protocol for genetic transformation of common bean has two additional advantages with respect to previous reports: (1) it allows for obtaining transformed regenerants and (2) the genetic transformation was stable for the selective gene.

Article

COMMON BEAN RECALCITRANT SPECIES GENETIC TRANSFORMATION A. TUMEFACIENS GUSA ACTIVIT BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR DE PLANTAS BIOLOGÍA MOLECULAR DE PLANTAS

Proteomic analysis of amaranth (Amaranthus hypochondriacus L.) leaves under drought stress

JOSE ANGEL HUERTA OCAMPO ERIKA PATRICIA BRIONES CERECERO ANTONIO DE LEON RODRIGUEZ ANA PAULINA BARBA DE LA ROSA (2009)

"Amaranth (Amaranthus hypochondriacus L.) is a plant that produces seed with high protein content, is rich on nutraceutical compounds, and can grow under environmental conditions where most of the basic crops are not able to develop. But little is know about the amaranth stress-responsive genes/proteins. The aim of this work was to apply the comparative proteomics approach to study the differential expression of amaranth leaf proteins under drought stress. However, the protein extraction from amaranth tissues is difficult as a result of high endogenous concentrations of interfering compounds; we have made some modifications of the classical trichloroacetic acid-acetone precipitation method to improve the quantity and quality of extracted proteins. Satisfactory and reproducible two-dimensional electrophoresis protein profiles were obtained; the method was also tested forAgave tequilana and Opuntia spp., two more examples of plants that are tolerant to drought stress. Drought-responsive proteins in amaranth leaves were identified by liquid chromatography electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS). The upregulated proteins identified included chloroplast chaperonins involved in refolding and protein complexes protection. Downregulated proteins include Rubisco large subunit, cytochrome b6f, oxygen evolving complexes, and the ascorbate peroxidase mitochondrial. The results have shown that chloroplasts and mitochondria may play a central role in amaranth adaptation to abiotic stress, and further studies should be done at the subcellular level."

Article

Abiotic stress Agave LC/ESI‐MS/MS Nonmodel plants, Opuntia spp Recalcitrant plants BIOLOGÍA Y QUÍMICA