Título
Optimization of analytical conditions to determine steroids and pharmaceuticals drugs in water sample using solid phase-extraction and HPLC
Autor
ALBERTO LOPEZ LOPEZ
LEONEL HERNANDEZ MENA
RAMIRO VALLEJO RODRIGUEZ
MARIO ALFONSO MURILLO TOVAR
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: doi:10.4236/ajac.2011.28099
Materias
Resumen o descripción
Two reliable methods were optimized to determine two steroids (17-Estradiol and 17-Ethinylestradiol) and two pharmaceutical drugs (ibuprofen and naproxen) using Solid-Phase Extraction (SPE) for sample preparation and High Performance Liquid Chromatography (HPLC) for analysis. SPE (C18) conditions were evaluated varying elution solvent volume, pH conditions and sample mass in the cartridge and reduction techniques of the extract. The efficiency of the analytical methods was evaluated by spiking ultrapure water samples with compounds at three and four levels of concentration for steroids and pharmaceutical drugs, re- spectively. The recoveries were independent (P > 0.05) of added mass of target analytes with a repeatability lower than 6.5% for steroids and 12.1% for pharmaceutical compounds. The recovery factor (coefficient of variation, CV) was higher than 83% for steroids (CV < 3.8%) and >93% for pharmaceuticals (CV < 5.2%). The optimized analytical method was applied for the evaluation of a steroid degradation test using ozone, finding that the estimated limit of detection is sufficient to determine the residual mass (μg·L–1) of 17β-Estradiol after the experiment.
Editor
Scientific Research
Fecha de publicación
diciembre de 2011
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
American Journal of Analytical Chemistry
Idioma
Inglés
Audiencia
Estudiantes
Investigadores
Repositorio Orígen
Repositorio Institucional de CIATEJ
Descargas
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