Título

Cytotoxic effect of Spirulina platensis extracts on human acute leukemia Kasumi-1 and chronic myelogenous leukemia K-562 cell lines.

Autor

Sanghamitra Khandual

FLOR YOHANA FLORES HERNANDEZ

INOCENCIA GUADALUPE RAMIREZ LOPEZ

Nivel de Acceso

Acceso Abierto

Referencia de datos

Resumen o descripción

"Objective: To evaluate the cytotoxic effects of Spirulina platensis extracts on acute

leukemia Kasumi-1 and chronic leukemia K-562 cancer cell lines.

Methods: Various concentrations of Spirulina platensis extracts (0.25–50.00 mg/mL)

obtained with different solvents were used to treat cell lines for 72 h. For cytotoxic effect

studies, cell viability test with trypan blue solution, MTT assay and microscopic cytomorphological

assessment were done.

Results: Spirulina extract obtained with 70% ethanol showed significant cytotoxicity in

K562 and Kasumi-1 cell lines. With trypan blue solution, IC50 values were found to be

4.64 mg/mL for K-562 and 3.68 mg/mL for Kusumi-1 cell lines. Spirulina aqueous

extract also showed cytotoxicity with trypan blue method, at a slightly higher dose; where

IC50 values were 12.68 mg/mL for K-562 and 2.13 mg/mL for Kusumi-1 cell lines. The

IC50 values were found 0.40 mg/mL for K-562 and 0.31 mg/mL for Kusumi-1 cell lines

for the 70% ethanol extract according to the MTT assay. Spirulina extract obtained with

water also showed cytotoxicity but the dose was a little higher where IC50 values were

15.77 mg/mL for K-562 and 9.44 mg/mL for Kusumi-1 cell lines. The effect of cytotoxicity

with ethanol extract is quite comparable with that observed for cyclophosphamide,

which is a chemical used as anticancer agent.

Conclusions: The cytotoxicity exhibited by Spirulina extract to cancer cell lines might

be due to the presence of phytopigments (carotenoids, chlorophyll, phycocyanin) as well

as polysaccharides that were reported previously as constituents of the extract. So crude

extracts of Spirulina can be used as a source to develop anticancer drugs".

Editor

Elsevier B.V.

Fecha de publicación

23 de noviembre de 2016

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Formato

application/pdf

Idioma

Inglés

Audiencia

Estudiantes

Investigadores

Repositorio Orígen

Repositorio Institucional de CIATEJ

Descargas

567

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