Título
Development of a mucoadhesive buccal film as a carrier of Lippia graveolens oil emulsions and its in vitro evaluation against Candida albicans
Autor
ROSA JARUMY LOPEZ RIVERA
Colaborador
HUGO ESPINOSA ANDREWS (Asesor de tesis)
Nivel de Acceso
Acceso Abierto
Resumen o descripción
The purpose of this work is to develop a mucoadhesive film that serves as a carrier for Lippia
graveolens essential oil (LGO) emulsion and to evaluate in vitro their inhibitory effect against
opportunistic pathogen Candida. albicans. For this, different ratio Chitosan: Pectin (100:0,
75:25, 50:50, 25:75, 0:100) films were obtained by casting technique with different
concentration of glycerol as a plasticizer (0, 10 and 30%). Water-uptake, erosion, and
mucoadhesion were evaluated.
Films with CH:P ratio of 100:0 with 30% glycerol, 75:25 with 10% glycerol and 50:50 with
10% glycerol showed better mucoadhesion and physical properties. Characterization of LGO
was carried out, and major components thymol and carvacrol were quantified by GC-MS, these
phenols were present in a 2:1 ratio. Three different emulsions with LGO were developed and
characterized, their inhibitory effect was evaluated against C. albicans. Arabic gum (GA),
Hydroxylated lecithin (LecH) and polysorbate 80 (T80) were used as emulsifiers. The particle
size of emulsions was 1597±11.1, 179±5.5 and 137.3±4.2 respectively.
All emulsions had an inhibitory effect against C. albicans. Volumes of emulsions that inhibit
the 99% of C. albicans were used for the in vitro test of films together with emulsion.
With this work it was possible to develop three different emulsions with an inhibitory effect
against C. albicans, likewise, these emulsions showed an inhibitory effect in a localized way
when evaluated together with the mucoadhesive films against C. albicans
Fecha de publicación
12 de noviembre de 2017
Tipo de publicación
Tesis de maestría
Recurso de información
Formato
application/pdf
Repositorio Orígen
Repositorio Institucional de CIATEJ
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