Título

Detection of dengue, west Nile virus, rickettsiosis and leptospirosis by a new real-time PCR strategy

Autor

DARWIN EDUARDO ELIZONDO QUIROGA

ALBERTINA CARDENAS VARGAS

ERIKA NAHOMY MARINO MARMOLEJO

ABEL GUTIERREZ ORTEGA

ESTEBAN GONZALEZ DIAZ

MARIA DEL RAYO MORFIN OTERO

EDUARDO RODRIGUEZ NORIEGA

DANIEL GARCIA RUIZ

MARCO ALONSO MARTINEZ GUZMAN

HECTOR RAUL PEREZ GOMEZ

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Referencia de datos

doi: 10.1186/s40064-016-2318-y

Materias

BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) VIROLOGÍA - (CTI) ARBOVIRUS - (CTI)

Resumen o descripción

"Dengue virus (DENV) infection causes sudden fever along with several nonspecific signs and symptoms and in severe cases, death. DENV is transmitted to people by Aedes aegypti and Ae. albopictus mosquitoes, whose populations increase during rainy season. West Nile Virus (WNV), Rickettsia spp. and Leptospira spp. are fever-causing pathogens that share many of the initial symptoms of DENV infection and also thrive in the rainy season. Outbreaks in some regions may be due to any of these pathogens that can co-circulate. Plus, they are clinically indistinguishable until severe symptoms appear, even though these diseases should be treated differently. An effective differential diagnosis would help clinicians and vector control departments to make right decisions for control and treatment of these diseases. Therefore, we developed four different SYBR green®-based reverse transcription quantitative PCR (RT-qPCR) assays for simultaneous detection of DENV, WNV, Rickettsia spp. and Leptospira spp. The assay has been optimized to yield results in less than 1 h; and in order to reduce contamination risk, all reagents were premixed and lyophilized on 96 well plates and thus only requires the addition of water and total nucleic acids from the sample. Sensitivities of the assays were less than 100 copies of nucleic acid targeted for these four pathogens. Assays did not show cross reactivity with any of the four pathogens nor to human nucleic acids. We are presenting a sensitive and selective kit that detects four relevant pathogens from tropical regions, that is quick, cost-effective and easy to use."

Editor

Springer

Fecha de publicación

20 de mayo de 2016

Tipo de publicación

Artículo

Recurso de información

http://ciatej.repositorioinstitucional.mx/jspui/handle/1023/501

Formato

application/pdf

Fuente

Springer Plus

Idioma

Inglés

Audiencia

Estudiantes

Investigadores

Maestros

Medios de comunicación

Repositorio Orígen

Repositorio Institucional de CIATEJ

Descargas

728