Título
Ascorbic acid, ultraviolet C rays, and glucose but not hyperthermia are elicitors of human beta defensin 1 mRNA in normal keratinocytes
Autor
LUIS ANTONIO CRUZ DIAZ
MARIA GUADALUPE FLORES MIRAMONTES
PAULINA CHAVEZ HURTADO
Kirk Allen
MARISELA GONZALEZ AVILA
ERNESTO PRADO MONTES DE OCA
Nivel de Acceso
Acceso Abierto
Referencia de datos
doi: http://dx.doi.org/10.1155/2015/714580
Resumen o descripción
"Hosts’ innate defense systems are upregulated by antimicrobial peptide elicitors (APEs). Our aim was to investigate the effects of
hyperthermia, ultraviolet A rays (UVA), and ultraviolet C rays (UVC) as well as glucose and ascorbic acid (AA) on the regulation
of human 𝛽-defensin 1 (DEFB1), cathelicidin (CAMP), and interferon-𝛾 (IFNG) genes in normal human keratinocytes (NHK).
The indirect in vitro antimicrobial activity against Staphylococcus aureus and Listeria monocytogenes of these potential APEs was
tested. We found that AA is a more potent APE for DEFB1 than glucose in NHK. Glucose but not AA is an APE for CAMP. Mild
hypo- (35∘ C) and hyperthermia (39∘C) are not APEs in NHK. AA-dependent DEFB1 upregulation below 20 mM predicts in vitro
antimicrobial activity as well as glucose- and AA-dependent CAMP and IFNG upregulation. UVC upregulates CAMP and DEFB1
genes but UVA only upregulates the DEFB1 gene. UVC is a previously unrecognized APE in human cells. Our results suggest that
glucose upregulates CAMP in an IFN-𝛾-independent manner. AA is an elicitor of innate immunity that will challenge the current
concept of late activation of adaptive immunity of this vitamin. These results could be useful in designing new potential drugs and
devices to combat skin infections."
Editor
Hindawi Publishing Corporation
Fecha de publicación
2015
Tipo de publicación
Artículo
Recurso de información
Formato
application/pdf
Fuente
BioMed Research International Volume 2015, Article ID 714580, 9 pages
Idioma
Inglés
Audiencia
Bibliotecarios
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Investigadores
Maestros
Repositorio Orígen
Repositorio Institucional de CIATEJ
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