Título
Molecular Cloning and Variability of a Subolesin Recombinant Peptide from a Mexican Rhipicephalus (Boophilus) Microplus Tick Strain
Autor
RODOLFO ESTEBAN LAGUNES QUINTANILLA
DELIA INES DOMINGUEZ GARCIA
MOISES MARTINEZ VELAZQUEZ
HECTOR MANELIC QUIROZ ROMERO
RODRIGO ROSARIO CRUZ
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: DOI: 10.12966/bmb.06.01.2014
Resumen o descripción
"The cattle tick Rhipicephalus (Boophilus) microplus is the most important health problem for livestock at tropical and subtropical areas. Because of its hematofagous behavior, pathogen transmission, acaricide resistance, environmental impact and public health, new strategies for tick control are required. Vaccines, constitute a cost-effective and environment friendly alter-native. The Bm86 tick antigen, originally identified in R. microplus, is the constituent of the only commercialized anti-tick vaccine. Nowadays, tick subolesin has been considered an important candidate. The aim of this study was to clone and charac-terize a recombinant peptide derived from the gen Sub in order to express it in Escherichia coli. The recombinant peptide was labeled with a polihistidine tag and identified by a monoclonal antibody against the tag after PAGE separation, purified by Ni affinity chromatography, and analyzed by the cluster analysis of variations with regards to ten different tick strains including Mexican ticks. Analysis of variations show, different clusters suggesting regional specific variations among Mexican strains, with probable implications on regional variability on protection efficiency. The tick protective antigen, subolesin, is a promising antigen and the use of immunogenic peptides predicted from in silico analysis approached in this study can be an alternative to improve the efficiency of tick vaccines in the future.".
Editor
Sciknow Publications Ltd.
Fecha de publicación
2014
Tipo de publicación
Artículo
Recurso de información
Formato
application/pdf
Fuente
Biochemistry
&
Molecular Biology (2014) 17-23
Idioma
Inglés
Repositorio Orígen
Repositorio Institucional de CIATEJ
Descargas
363