Título
Transcriptome analysis of Catarina scallop (Argopecten ventricosus) juveniles treated with highly-diluted immunomodulatory compounds reveals activation of non-selfrecognition system
Autor
Jesús Antonio López Carvallo
JOSE MANUEL MAZON SUASTEGUI
MIGUEL ANGEL HERNANDEZ OÑATE
Dariel Tovar Ramírez
Fernando Abasolo Pacheco
ROSA MARIA MORELOS CASTRO
Guadalupe Fabiola Arcos Ortega
Nivel de Acceso
Acceso Abierto
Referencia de publicación
doi: DOI: 10.1371/journal.pone.0233064
URL/URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0233064
ISSN/ISSN: 1932-6203
Materias
Resumen o descripción
"Marine bivalve hatchery productivity is continuously challenged by apparition and propagation of new diseases, mainly those related to vibriosis. Disinfectants and antibiotics are frequently overused to prevent pathogen presence, generating a potential negative impact on the environment. Recently, the use of highly diluted compounds with immunostimulant properties in marine organisms has been trailed successfully to activate the self-protection mechanisms of marine bivalves. Despite their potential as immunostimulants, little is known about their way of action. To understand their effect, a comparative transcriptomic análisis was performed with Argopecten ventricosus juveniles. The experimental design consisted of four treatments formulated from pathogenic Vibrio lysates at two dilutions: [(T1) Vibrio parahaemolyticus and Vibrio alginolyticus 1D; (T2) V. parahaemolyticus and V. alginolyticus 7C]; minerals [(T3) PhA+SiT 7C], scorpion venom [(T4) ViT 31C]; and one control (C1) hydro-alcoholic solution (ethanol 1%). The RNA sequencing (RNAseq) analysis showed a higher modulation of differentially expressed genes (DEG) in mantle tissue compared to gill tissue. The scallops that showed a higher number of DEG related to immune response in mantle tissue corresponded to T1 (V. parahaemolyticus and V. alginolyticus lysate) and T3 (Silicea terra® - Phosphoric acid®). The transcriptome analysis allowed understanding some interactions between A. ventricosus juveniles and highly-diluted treatments."
Editor
Public Library of Science
Fecha de publicación
2020
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
PLoS ONE
Idioma
Inglés
Sugerencia de citación
López-Carvallo JA, Mazón-Suástegui JM, Hernández-Oñate MA, Tovar-Ramírez D, Abasolo- Pacheco F, Morelos-Castro RM, et al. (2020) Transcriptome analysis of Catarina scallop (Argopecten ventricosus) juveniles treated with highly-diluted immunomodulatory compounds reveals activation of non-self-recognition system. PLoS ONE 15(5): e0233064. https://doi.org/ 10.1371/journal.pone.0233064
Repositorio Orígen
Repositorio Institucional CIBNOR
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