Título

Transcriptome analysis of Catarina scallop (Argopecten ventricosus) juveniles treated with highly-diluted immunomodulatory compounds reveals activation of non-selfrecognition system

Autor

Jesús Antonio López Carvallo

JOSE MANUEL MAZON SUASTEGUI

MIGUEL ANGEL HERNANDEZ OÑATE

Dariel Tovar Ramírez

Fernando Abasolo Pacheco

ROSA MARIA MORELOS CASTRO

Guadalupe Fabiola Arcos Ortega

Nivel de Acceso

Acceso Abierto

Referencia de publicación

doi: DOI: 10.1371/journal.pone.0233064

URL/URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0233064

ISSN/ISSN: 1932-6203

Resumen o descripción

"Marine bivalve hatchery productivity is continuously challenged by apparition and propagation of new diseases, mainly those related to vibriosis. Disinfectants and antibiotics are frequently overused to prevent pathogen presence, generating a potential negative impact on the environment. Recently, the use of highly diluted compounds with immunostimulant properties in marine organisms has been trailed successfully to activate the self-protection mechanisms of marine bivalves. Despite their potential as immunostimulants, little is known about their way of action. To understand their effect, a comparative transcriptomic análisis was performed with Argopecten ventricosus juveniles. The experimental design consisted of four treatments formulated from pathogenic Vibrio lysates at two dilutions: [(T1) Vibrio parahaemolyticus and Vibrio alginolyticus 1D; (T2) V. parahaemolyticus and V. alginolyticus 7C]; minerals [(T3) PhA+SiT 7C], scorpion venom [(T4) ViT 31C]; and one control (C1) hydro-alcoholic solution (ethanol 1%). The RNA sequencing (RNAseq) analysis showed a higher modulation of differentially expressed genes (DEG) in mantle tissue compared to gill tissue. The scallops that showed a higher number of DEG related to immune response in mantle tissue corresponded to T1 (V. parahaemolyticus and V. alginolyticus lysate) and T3 (Silicea terra® - Phosphoric acid®). The transcriptome analysis allowed understanding some interactions between A. ventricosus juveniles and highly-diluted treatments."

Editor

Public Library of Science

Fecha de publicación

2020

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Formato

application/pdf

Fuente

PLoS ONE

Idioma

Inglés

Sugerencia de citación

López-Carvallo JA, Mazón-Suástegui JM, Hernández-Oñate MA, Tovar-Ramírez D, Abasolo- Pacheco F, Morelos-Castro RM, et al. (2020) Transcriptome analysis of Catarina scallop (Argopecten ventricosus) juveniles treated with highly-diluted immunomodulatory compounds reveals activation of non-self-recognition system. PLoS ONE 15(5): e0233064. https://doi.org/ 10.1371/journal.pone.0233064

Repositorio Orígen

Repositorio Institucional CIBNOR

Descargas

237

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