Título
Algevir: An Expression System for Microalgae Based on Viral Vectors
Autor
BERNARDO BAÑUELOS HERNANDEZ
ELIZABETH MONREAL ESCALANTE
OMAR GONZALEZ ORTEGA
CARLOS ELIUD ANGULO VALADEZ
SERGIO ROSALES MENDOZA
Nivel de Acceso
Acceso Abierto
Referencia de publicación
doi: DOI: 10.3389/fmicb.2017.01100
ISSN/ISSN: 1664-302X
URL/URL: https://www.frontiersin.org/journals/microbiology
Materias
Resumen o descripción
"The use of recombinant algae for the production of valuable compounds is opening promising biotechnological applications. However, the development of efficient expression approaches is still needed to expand the exploitation of microalgae in biotechnology. Herein, the concept of using viral expression vectors in microalgae was explored for the first time. An inducible geminiviral vector leading to Rep-mediated replication of the expression cassette allowed the production of antigenic proteins at high levels. This system, called Algevir, allows the production of complex viral proteins (GP1 from Zaire ebolavirus) and bacterial toxin subunits (B subunit of the heat-labile Escherichia coli enterotoxin), which retained their antigenic activity. The highest achieved yield was 1.25 mg/g fresh biomass (6 mg/L of culture), which was attained 3 days after transformation. The Algevir system allows for a fast and efficient production of recombinant proteins, overcoming the difficulties imposed by the low yields and unstable expression patterns frequently observed in stably transformed microalgae at the nuclear level; as well as the toxicity of some target proteins."
Editor
Frontiers Media, S. A.
Fecha de publicación
2017
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
Frontiers in Microbiology
Idioma
Inglés
Sugerencia de citación
Bañuelos-Hernández B, Monreal-Escalante E, González-Ortega O, Angulo C and Rosales-Mendoza S (2017) Algevir: An Expression System for Microalgae Based on Viral Vectors. Front. Microbiol. 8:1100.
Repositorio Orígen
Repositorio Institucional CIBNOR
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