Título

Molecular characterization of Yucatan tomato phytoplasma (Group 16Sr III)

Autor

RAUL TAPIA TUSSELL

ALBA PRISCILIA SUASTE DZUL

ALBERTO CORTES VELAZQUEZ

CLAUDIA GUADALUPE TORRES CALZADA

ANDRES FELIPE DE JESUS QUIJANO RAMAYO

RODOLFO MARTIN MEX

ANGEL NEXTICAPAN GARCEZ

DAISY DE LA CARIDAD PEREZ BRITO

Nivel de Acceso

Acceso Abierto

Referencia de datos

doi: DOI: 10.5897/AJB11.3105

Resumen o descripción

Tomato (Lycopersicon esculentum) is an important vegetable crop in Mexico. Recently, a phytoplasma associated with leaf yellowing and curling, severe stunting and little leaf in tomato plant was identified as Yucatan tomato phytoplasma (16SrIII group). DNAs extracted from tomato leaves with symptoms were examined for the presence of this phytoplasma by nested polymerase chain reaction (PCR). Positive results were obtained in 44% of samples, yielding an rDNA product of 1.25 kb. In vitro and in silico restriction fragment length polymorphism (RFLP) patterns obtained with endonucleases HpaII, MseI, RsaI and TaqI were characteristics of group 16SrIII, according to the classification scheme of phytoplasmas. The pattern with AluI and HaeIII discriminated between these phytoplasmas and the members of 16SrIII group. Molecular characterization of the causal agent of Yucatan tomato phytoplasma will facilitate the study of this disease’s epidemic aspects and its phytosanitary management. In addition, it will contribute to a greater knowledge of the genetic diversity of phytoplasmas present in Mexico.

Fecha de publicación

2012

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Formato

application/pdf

Fuente

African Journal of Biotechnology, 11(9), 2169-2177, 2012

Idioma

Inglés

Relación

&

Perez-Brito, D. (2012). Molecular characterization of Yucatan tomato phytoplasma (Group 16Sr III). African Journal of Biotechnology, 11(9), 2169-2177.

Sugerencia de citación

Tapia-Tussell, R., Suaste-Dzul, A., Cortes-Velazquez, A., Torres-Calzada, C., Quijano-Ramayo, A., Martin-Mex, R., ...

Repositorio Orígen

Repositorio Institucional CICY

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291

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