Título
Molecular characterization of Yucatan tomato phytoplasma (Group 16Sr III)
Autor
RAUL TAPIA TUSSELL
ALBA PRISCILIA SUASTE DZUL
ALBERTO CORTES VELAZQUEZ
CLAUDIA GUADALUPE TORRES CALZADA
ANDRES FELIPE DE JESUS QUIJANO RAMAYO
RODOLFO MARTIN MEX
ANGEL NEXTICAPAN GARCEZ
DAISY DE LA CARIDAD PEREZ BRITO
Nivel de Acceso
Acceso Abierto
Referencia de datos
doi: DOI: 10.5897/AJB11.3105
Materias
Resumen o descripción
Tomato (Lycopersicon esculentum) is an important vegetable crop in Mexico. Recently, a phytoplasma associated with leaf yellowing and curling, severe stunting and little leaf in tomato plant was identified as Yucatan tomato phytoplasma (16SrIII group). DNAs extracted from tomato leaves with symptoms were examined for the presence of this phytoplasma by nested polymerase chain reaction (PCR). Positive results were obtained in 44% of samples, yielding an rDNA product of 1.25 kb. In vitro and in silico restriction fragment length polymorphism (RFLP) patterns obtained with endonucleases HpaII, MseI, RsaI and TaqI were characteristics of group 16SrIII, according to the classification scheme of phytoplasmas. The pattern with AluI and HaeIII discriminated between these phytoplasmas and the members of 16SrIII group. Molecular characterization of the causal agent of Yucatan tomato phytoplasma will facilitate the study of this disease’s epidemic aspects and its phytosanitary management. In addition, it will contribute to a greater knowledge of the genetic diversity of phytoplasmas present in Mexico.
Fecha de publicación
2012
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
African Journal of Biotechnology, 11(9), 2169-2177, 2012
Idioma
Inglés
Relación
&
Perez-Brito, D. (2012). Molecular characterization of Yucatan tomato phytoplasma (Group 16Sr III). African Journal of Biotechnology, 11(9), 2169-2177.
Sugerencia de citación
Tapia-Tussell, R., Suaste-Dzul, A., Cortes-Velazquez, A., Torres-Calzada, C., Quijano-Ramayo, A., Martin-Mex, R., ...
Repositorio Orígen
Repositorio Institucional CICY
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