Título
Efficient plant regeneration from leaf explants of Solanum americanum
Autor
INGRID AILEEN O'CONNOR SANCHEZ
ANGEL VIRGILIO DOMINGUEZ MAY
MIGUEL ANGEL KEB LLANES
TOMAS AUGUSTO GONZALEZ ESTRADA
YURI JORGE JESUS PEÑA RAMIREZ
Nivel de Acceso
Acceso Abierto
Materias
Resumen o descripción
A very efficient system for direct plant regeneration from in vitro-derived leaf explants of Solanum americanum was developed. S. americanum is a tropical plant with important medical properties. The in vitro procedure that was established consists of (i) induction of shoots from leaf tissue, (ii) elongation of shoots, and (iii) rooting of plantlets. The induction of shoots was achieved on Murashige and Skoog solid medium supplemented with different combinations of zeatin riboside and 1-naphthalene acetic acid or 6-benzylaminopurine and 1-naphthalene acetic acid. The best combination for plant regeneration was MS with 5.7 μM zeatin riboside and 0.11 μM 1-naphthalene acetic acid. In the second step, the shoot clumps were transferred to MS basal medium without plant growth regulators, resulting in the differentiation of most of the shoot initials into well developed shoots. In the third stage, plantlets were efficiently rooted on half-strength MS basal medium supplemented with 58.5 mM sucrose. The rooted plants were established in soil with a 100% success rate. This system can be useful to perform further experiments to obtain transgenic plants of this species as well as for other biotechnological approaches. © 2010 Academic Journals.
Fecha de publicación
2010
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
African Journal of Biotechnology, 9(36), 5830-5835, 2010
Idioma
Inglés
Relación
&
Peña-Ramírez, Y. J. (2010). Efficient plant regeneration from leaf explants of solanum americanum. African Journal of Biotechnology, 9(36), 5830-5835.
Sugerencia de citación
O'Connor-Sánchez, A., Domínguez-May, A. V., Keb-Llanes, M. A., González-Estrada, T. A.,
Repositorio Orígen
Repositorio Institucional CICY
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