Título
Endogenous GUS-like activity in Capsicum chinense Jacq.
Autor
LAURA YESENIA SOLÍS RAMOS
TOMAS AUGUSTO GONZALEZ ESTRADA
ANTONIO ANDRADE TORRES
GREGORIO DEL CARMEN GODOY HERNANDEZ
ENRIQUE CASTAÑO DE LA SERNA
Nivel de Acceso
Acceso Abierto
Referencia de datos
datasetDOI/DOI: 10.2225/vol13-issue4-fulltext-3
Materias
Resumen o descripción
The gene uidA, codes for β-glucuronidase, which is one of the reporters more frequently utilized in transgenic plants. However, this can only be use if the selected organism does not present endogenous GUS-like activity. In tissues of C. chinense we found a GUS-like activity showing different levels of intensity. Histochemical screening showed that endogenous GUS-like activitydecreased, or reduced significantly, in almost all tissues with exception of stament, when phosphate buffer was adjusted to pH 8. Subsequently, C. chinense zygotic embryo explants were transient transformed with Agrobacterium tumefaciens LBA4404 (pCAMBIA2301) and plantlets regenerated were histochemically stained in phosphate buffer pH 8. Observations of incubated tissues of C. chinense regenerants showed blue staining, suggesting expression of uidA. Incubated tissues of non-transformed regenerants did not show blue staining in phosphate buffer pH 8. The results show that for transformation experiments of C. chinense with uidA gene, pH 8 is recommended for histochemical staining.
Fecha de publicación
2010
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
Electronic Journal of Biotechnology, 13(4), 2-3, 2010
Idioma
Inglés
Relación
&
Castaño de la Serna, E. (2010). Endogenous GUS-like activity in Capsicum chinense Jacq. Electronic Journal of Biotechnology, 13(4), 2-3.
Sugerencia de citación
Solís-Ramos, L. Y., González-Estrada, T., Andrade-Torres, A., Godoy-Hernández, G.,
Repositorio Orígen
Repositorio Institucional CICY
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