Título

Protein extraction for Cocos nucifera with and without lethal yellowing phytoplasma: gel-based proteomics

Autor

CELSO REYES MARTINEZ

DANAE MORALES ANGELES

MARIA DEL SOCORRO NARVAEZ CAB

JUAN ALBERTO OSUNA CASTRO

DANIEL ZIZUMBO VILLARREAL

CARLOS MARIANO OROPEZA SALIN

ELPIDIO PEÑA BELTRAN

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Materias

COCOS NUCIFERA - (AUTORES) PHYTOPLASMA - (AUTORES) PHENOLS - (AUTORES) PROTEOMICS - (AUTORES) ECOTYPES - (AUTORES) GEL ELECTROPHORESIS - (AUTORES) PLANT EXTRACTS - (AUTORES) METHANOL - (AUTORES) PIGMENTS - (AUTORES) BIOLOGÍA Y QUÍMICA - (CTI) BIOLOGÍA Y QUÍMICA - (CTI)

Resumen o descripción

Two-dimensional gel electrophoresis (2-DE) is one of the two methodologies to separate and identify gene expression at the proteome level. However, the separation of proteins from plant tissue extract is often complicate, because it is necessary to modify the protocols depending upon the type of tissue and upon the presence of different interfering compounds. In this work it is established a routine procedure for the application of proteomic analysis to study interaction between palm coconut and lethal yellowing (LY) phytoplasma. The characteristics of protocol include: (i) cleanup of pigments and contaminants of the trunk palm coconut tissue, using a solution of acetone with TCA and 2-mercaptoethanol. (ii) Phenol extraction of proteins in presence of methanol with ammonium acetate. 2-DE analysis revealed differences in palm coconut with and without LY agent and similitude between palm ecotypes.

Fecha de publicación

2007

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1215

Formato

application/pdf

Fuente

Bulletin of Insectology, 60(2), 231-232, 2007.

Idioma

Inglés

Relación

&

Pena-Beltran, E. (2007). Protein extraction for Cocos nucifera with and without lethal yellowing phytoplasma: gel-based proteomics. Bulletin of Insectology, 60(2), 231-232.

Sugerencia de citación

Reyes-Martinez, C., Morales-Angeles, D., Narvaez-Cab, M., Osuna-Castro, J. A., Zizumbo-Villarreal, D., Oropeza-Salin, C. M.,

Repositorio Orígen

Repositorio Institucional CICY

Descargas

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