Título
Genetic variability in Rhizoctonia solani isolated from Vitis vinifera based on amplified fragment length polymorphism
Autor
AMPARO MEZA MOLLER
MARTIN CANDELARIO ESQUEDA VALLE
LORENZO FELIPE SANCHEZ TEYER
GEORGINA VARGAS ROSALES
ALFONSO ANTERO GARDEA BEJAR
MARTIN ERNESTO TIZNADO HERNANDEZ
Nivel de Acceso
Acceso Abierto
Referencia de publicación
doi: 10.3844/ajabssp.2011.317.323
Referencia de datos
datasetDOI/10.3844/ajabssp.2011.317.323
Materias
RHIZOCTONIA SOLANI - (AUTORES) THANATEPHORUS CUCUMERIS - (AUTORES) MOLECULAR MARKERS - (AUTORES) VINEYARDS - (AUTORES) GENETIC VARIABILITY - (AUTORES) AMPLIFIED FRAGMENT LENGTH POLYMORPHISM - (AUTORES) GRAPEVINE PATHOGEN - (AUTORES) PERLETTE SEEDLESS - (AUTORES) SIMILARITY INDEXES - (AUTORES) BIOLOGÍA Y QUÍMICA - (CTI) BIOLOGÍA Y QUÍMICA - (CTI)
Resumen o descripción
Problem statement: Rhizoctonia solani is a potential grapevine pathogen. In order to develop effective methods of control, it is necessary to document its genetic diversity. Approach: The objective of this study was to analyze the genetic variability of R. solani isolated from the rhizosphere of ungrafted V. vinifera var. perlette seedless planted in Sonora, Mexico using Amplified Fragment Length Polymorphism (AFLP). Results: In the selective amplification using eight primer combinations we obtained a total of 446 AFLP markers with a 100% polymorphism. Out of 41 isolates, 36 different AFLP patterns were observed and five were replicates of the same pattern. The dendrogram shows inter- and intrapopulation similarity indexes of 0.26, 0.98 and 0.31, 0.98, respectively. Six groups emerged from the principal components analysis, five of which were clearly defined, while the other one was spread out. Conclusion: We conclude that R. solanigrowing in Sonoran vineyards shows a high degree of genetic variability, even under similar environmental conditions.
Fecha de publicación
2011
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
American Journal of Agricultural and Biological Science. 6(3) 317-323, 2011.
Idioma
Español
Relación
&
Tiznado-Hernandez, M. (2011). Genetic variability in Rhizoctonia solani isolated from Vitis vinifera based on Amplified Fragment Length Polymorphism. American Journal of Agricultural and Biological Science. 6(3) 317-323.
Sugerencia de citación
Meza-Moller, A., Esqueda, M., Sanchez-Teyer, F., Vargas-Rosales, G., Gardea, A. A.,
Repositorio Orígen
Repositorio Institucional CICY
Descargas
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