Título

Capsaicin synthesis requires in situ phenylalanine and valine formation in in vitro maintained placentas from Capsicum chinense

Autor

MARIA DE LOURDES MIRANDA HAM

Colaborador

FRAY MARTIN BAAS ESPINOLA (Colaborador)

LIZBETH ARIANELLY CASTRO CONCHA (Colaborador)

FELIPE AUGUSTO VAZQUEZ FLOTA (Colaborador)

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Referencia de datos

doi: DOI: 10.3390/molecules21060799

Materias

SOLANACEAE - (AUTORES) HABANERO PEPPER - (AUTORES) INHIBITORS - (AUTORES) CHLORSULFURON - (AUTORES) P-FLUOROPHENYLALANINE - (AUTORES) BIOLOGÍA Y QUÍMICA - (CTI)

Resumen o descripción

Capsaicinoids (CAP) are nitrogenous metabolites formed from valine (Val) and phenylalanine (Phe) in the placentas of hot Capsicum genotypes. Placentas of Habanero peppers can incorporate inorganic nitrogen into amino acids and have the ability to secure the availability of the required amino acids for CAP biosynthesis. In order to determine the participation of the placental tissue as a supplier of these amino acids, the effects of blocking the synthesis of Val and Phe by using specific enzyme inhibitors were analyzed. Isolated placentas maintained in vitro were used to rule out external sources' participation. Blocking Phe synthesis, through the inhibition of arogenate dehydratase, significantly decreased CAP accumulation suggesting that at least part of Phe required in this process has to be produced in situ. Chlorsulfuron inhibition of acetolactate synthase, involved in Val synthesis, decreased not only Val accumulation but also that of CAP, pointing out that the requirement for this amino acid can also be fulfilled by this tissue. The presented data demonstrates that CAP accumulation in in vitro maintained placentas can be accomplished through the in situ availability of Val and Phe and suggests that the synthesis of the fatty acid chain moiety may be a limiting factor in the biosynthesis of these alkaloids.

Fecha de publicación

1 de junio de 2016

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://cicy.repositorioinstitucional.mx/jspui/handle/1003/494

Formato

application/pdf

Fuente

Molecules. 21(6), E799, 2016

Idioma

Inglés

Relación

&

Miranda-Ham, M. L. (2016). Capsaicin synthesis requires in situ phenylalanine and valine formation in in vitro maintained placentas from Capsicum chinense. Molecules, 21(6), 799.

Sugerencia de citación

Baas-Espinola, F. M., Castro-Concha, L. A., Vázquez-Flota, F. A.,

Repositorio Orígen

Repositorio Institucional CICY

Descargas

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