Título

Antibacterial activity of defensin PaDef from Avocado Fruit (Persea americana var. drymifolia) Expressed in endothelial cells against Escherichia coli and Staphylococcus aureus

Autor

JAQUELINA JULIA GUZMAN RODRIGUEZ

RODOLFO LOPEZ GOMEZ

RAFAEL SALGADO GARCIGLIA

LUIS CARLOS RODRIGUEZ ZAPATA

ALEJANDRA OCHOA ZARZOSA

JOEL EDMUNDO LOPEZ MEZA

Nivel de Acceso

Acceso Abierto

Referencia de datos

doi: DOI: 10.1155/2013/986273

Resumen o descripción

Antimicrobial therapy is a useful tool to control infectious diseases in general and rising antibiotic resistant microorganisms in particular. Alternative strategies are desirable, and antimicrobial peptides (AMP) represent attractive control agents. Mexican avocado (Persea americana var. drymifolia) is used in traditional medicine; however, the AMP production has not been reported in this plant. We obtained a cDNA library from avocado fruit and clone PaDef was identified, which has a cDNA (249 bp) encoding a protein (78 aa) homologous with plant defensins (>80%). We expressed the defensin PaDef cDNA (pBME3) in the bovine endothelial cell line BVE-E6E7. Polyclonal and clonal populations were obtained and their activity was evaluated against Escherichia coli, Staphylococcus aureus, and Candida albicans. E. coli viability was inhibited with 100 g/mL of total protein from clones (>55%). Also, S. aureus viability was inhibited from 50 g/mL total protein (27-38%) but was more evident at 100 g/mL (52-65%). This inhibition was higher than the effect showed by polyclonal population (23%). Finally, we did not detect activity against C. albicans. These results are the first report that shows antimicrobial activity of a defensin produced by avocado and suggest that this AMP could be used in the control of pathogens.

Fecha de publicación

2013

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Formato

application/pdf

Fuente

BioMed Research International, 2013 doi:10.1155/2013/986273

Idioma

Inglés

Relación

&

López-Meza, J. E. (2013). Antibacterial activity of defensin PaDef from avocado fruit (persea americana var. drymifolia) expressed in endothelial cells against escherichia coli and staphylococcus aureus. BioMed Research International, 2013 doi:10.1155/2013/986273

Sugerencia de citación

Guzmán-Rodríguez, J. J., López-Gómez, R., Suárez-Rodríguez, L. M., Salgado-Garciglia, R., Rodríguez-Zapata, L. C., Ochoa-Zarzosa, A.,

Repositorio Orígen

Repositorio Institucional CICY

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705

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