Título
Antibacterial activity of defensin PaDef from Avocado Fruit (Persea americana var. drymifolia) Expressed in endothelial cells against Escherichia coli and Staphylococcus aureus
Autor
JAQUELINA JULIA GUZMAN RODRIGUEZ
RODOLFO LOPEZ GOMEZ
RAFAEL SALGADO GARCIGLIA
LUIS CARLOS RODRIGUEZ ZAPATA
ALEJANDRA OCHOA ZARZOSA
JOEL EDMUNDO LOPEZ MEZA
Nivel de Acceso
Acceso Abierto
Referencia de datos
doi: DOI: 10.1155/2013/986273
Materias
Resumen o descripción
Antimicrobial therapy is a useful tool to control infectious diseases in general and rising antibiotic resistant microorganisms in particular. Alternative strategies are desirable, and antimicrobial peptides (AMP) represent attractive control agents. Mexican avocado (Persea americana var. drymifolia) is used in traditional medicine; however, the AMP production has not been reported in this plant. We obtained a cDNA library from avocado fruit and clone PaDef was identified, which has a cDNA (249 bp) encoding a protein (78 aa) homologous with plant defensins (>80%). We expressed the defensin PaDef cDNA (pBME3) in the bovine endothelial cell line BVE-E6E7. Polyclonal and clonal populations were obtained and their activity was evaluated against Escherichia coli, Staphylococcus aureus, and Candida albicans. E. coli viability was inhibited with 100 g/mL of total protein from clones (>55%). Also, S. aureus viability was inhibited from 50 g/mL total protein (27-38%) but was more evident at 100 g/mL (52-65%). This inhibition was higher than the effect showed by polyclonal population (23%). Finally, we did not detect activity against C. albicans. These results are the first report that shows antimicrobial activity of a defensin produced by avocado and suggest that this AMP could be used in the control of pathogens.
Fecha de publicación
2013
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
BioMed Research International, 2013 doi:10.1155/2013/986273
Idioma
Inglés
Relación
&
López-Meza, J. E. (2013). Antibacterial activity of defensin PaDef from avocado fruit (persea americana var. drymifolia) expressed in endothelial cells against escherichia coli and staphylococcus aureus. BioMed Research International, 2013 doi:10.1155/2013/986273
Sugerencia de citación
Guzmán-Rodríguez, J. J., López-Gómez, R., Suárez-Rodríguez, L. M., Salgado-Garciglia, R., Rodríguez-Zapata, L. C., Ochoa-Zarzosa, A.,
Repositorio Orígen
Repositorio Institucional CICY
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