Título

Development of a large set of microsatellite markers in zapote mamey (Pouteria sapota (Jacq.) H.E. Moore

&

Stearn) and their potential use in the study of the species

Autor

JAIME MARTINEZ CASTILLO

NASIB HIRAM BLANCARTE JASSO

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Referencia de datos

doi: DOI: 10.3390/molecules200611400

Materias

BLAST ANALYSIS - (AUTORES) FOUNDER EFFECT - (AUTORES) GENETIC DIVERSITY - (AUTORES) GERMPLASM - (AUTORES) DOMESTICATION - (AUTORES) BIOLOGÍA Y QUÍMICA - (CTI)

Resumen o descripción

Pouteria sapota is known for its edible fruits that contain unique carotenoids, as well as for its fungitoxic, anti-inflammatory and anti-oxidant activity. However, its genetics is mostly unknown, including aspects about its genetic diversity and domestication process. We did high-throughput sequencing of microsatellite-enriched libraries of P. sapota, generated 5223 contig DNA sequences, 1.8 Mbp, developed 368 microsatellites markers and tested them on 29 individuals from 10 populations (seven wild, three cultivated) from Mexico, its putative domestication center. Gene ontology BLAST analysis of the DNA sequences containing microsatellites showed potential association to physiological functions. Genetic diversity was slightly higher in cultivated than in the wild gene pool (HE = 0.41 and HE = 0.35, respectively), although modified Garza-Williamson Index and Bottleneck software showed evidence for a reduction in genetic diversity for the cultivated one. Neighbor Joining, 3D Principal Coordinates Analysis and assignment tests grouped most individuals according to their geographic origin but no clear separation was observed between wild or cultivated gene pools due to, perhaps, the existence of several admixed populations. The developed microsatellites have a great potential in genetic population and domestication studies of P. sapota but additional sampling will be necessary to better understand how the domestication process has impacted the genetic diversity of this fruit crop.

Fecha de publicación

1 de junio de 2015

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://cicy.repositorioinstitucional.mx/jspui/handle/1003/721

Formato

application/pdf

Fuente

Molecules. 20(6), 11400-11417, 2015

Idioma

Inglés

Relación

&

Scheffler, B. E. (2015). Development of a large set of microsatellite markers in zapote mamey (Pouteria sapota (Jacq.) HE Moore

&

Stearn) and their potential use in the study of the species. Molecules, 20(6), 11400-11417.

Sugerencia de citación

Arias, R. S., Martínez-Castillo, J., Sobolev, V. S., Blancarte-Jasso, N. H., Simpson, S. A., Ballard, L. L., ...

Repositorio Orígen

Repositorio Institucional CICY

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