Título
In vitro regeneration of Pinus brutia Ten. var. eldarica (Medw.) through organogenesis
Autor
JERICO JABIN BELLO BELLO
LOURDES GEORGINA IGLESIAS ANDREU
LAZARO RAFAEL SANCHEZ VELASQUEZ
NANCY SANTANA BUZZY
Nivel de Acceso
Acceso Abierto
Referencia de datos
doi: DOI: 10.5897/AJB12.2180
Materias
Resumen o descripción
This paper describes two in vitro regeneration systems through direct and indirect organogenesis in Pinus brutia using fascicles aseptic cultures as explants. Mechanical scarification and gibberellic acid (GA3) were evaluated on in vitro seed germination. Scarification was the treatment that allowed for in vitro seed germination. The highest direct organogenic response was obtained in Murashige and Skoog (MS) medium containing 4.5 µM thidiazuron, whereas the highest indirect organogenesis was obtained with 9.8 µM thidiazuron and 3.4 µM paclobutrazol. The isolated shoots were rooted on MS medium supplemented with 1.70 µM indoleacetic acid. A large variation in root ability was observed among plantlets. These results suggest that both regeneration systems can be applied to the micropropagation or genetic transformation of P. brutia.
Fecha de publicación
20 de noviembre de 2012
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
African Journal of Biotechnology, 11(93), 15982-15987, 2012
Idioma
Inglés
Relación
&
Santana-Buzzy, N. (2012). In vitro regeneration of Pinus brutia Ten. var. eldarica (Medw.) through organogenesis. African Journal of Biotechnology, 11(93), 15982-15987.
Sugerencia de citación
Bello-Bello, J., Iglesias-Andreu, L., Sánchez-Velásquez, L., Casas-Martínez, J.,
Repositorio Orígen
Repositorio Institucional CICY
Descargas
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