Título
Saccharification with Phanerochaete chrysosporium and Pleurotus ostreatus enzymatic extracts of pretreated banana waste
Autor
XENIA MENA ESPINO
LUIS FELIPE BARAHONA PEREZ
LILIANA MARIA ALZATE GAVIRIA
REFUGIO RODRIGUEZ VAZQUEZ
MIGUEL ALONSO TZEC SIMA
JORGE ARTURO DOMINGUEZ MALDONADO
BLONDY BEATRIZ CANTO CANCHE
Nivel de Acceso
Acceso Abierto
Materias
Resumen o descripción
Lignocellulosic biomass has a great potential as raw material for second and third generation biofuels since it is the most abundant carbohydrate on earth and the main component of agricultural waste; however, saccharification of lignocellulosic biomass is crucial for the establishment of a carbohydratebased economy. The use of fungal enzymes is the preferred procedure for lignocellulosic saccharification. Fungi such as basidiomycetes (e.g Phanerochaete chrysosporium) produce cellulolytic/hemicellulolytic and ligninolytic enzymes, which are responsible for lignocellulose degradation. In this study the saccharification of banana flour prepared from pseudostem and green non commercial-grade fruit (1:1), two of the main agro-waste of banana industry was investigated. The material was pretreated by physical and chemical processes including drying and grinding, followed by 3% HCl or 3% NaOH hydrolysis, or a sequential pretreatment with 3% HCl first and then 3% NaOH and heated at 121°C, at 15 Lb/in2 for 15 min. The highest concentration of reducing sugars (RS) was obtained with acid hydrolysis (42.41 gL-1). Crude cellulolytic-ligninolytic enzymatic extracts from Pleurotus ostreatus and P. chrysosporium cultured on banana waste as the only carbon source were prepared and used for the saccharification. Surprisingly, P. chrysosporium crude extract produced a decrease in RS (2.27 gL-1). Although P. ostreatus cellulose activity (17,777.78 UL-1) was almost half compared to P. chrysosporium's (31,296.30 UL-1), the former produced an increment in the release ofRS (63.65 gL-1). In Mexico, banana is one of the main crops and generates large agricultural waste after harvest. According to the results obtained with acid-heat pretreatment followed by saccharification with P. ostreatus enzymatic crude extract, banana agro-waste can be considered as a potential feedstock for RS-based bioproducts like bioethanol.
Fecha de publicación
2011
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
African Journal of Biotechnology, 10(19), 3824-3834, 2011
Idioma
Inglés
Relación
&
Canto-Canché, B. B. (2011). Saccharification with phanerochaete chrysosporium and pleurotus ostreatus enzymatic extracts of pretreated banana waste. African Journal of Biotechnology, 10(19), 3824-3834.
Sugerencia de citación
Mena-Espino, X., Barahona-Pérez, F., Alzate-Gaviria, L., Rodríguez-Vázquez, R., Tzec-Simá, M., Domínguez-Maldonado, J.,
Repositorio Orígen
Repositorio Institucional CICY
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