Título

Encapsulación de embriones somáticos de Laelia anceps ssp. dawsonii para la producción de semilla sintética

Somatic embryoids encapsulation of Laelia anceps ssp. dawsonii to produce synthetic seed

Autor

HILDA EULALIA LEE ESPINOSA

JOAQUIN MURGUIA GONZALEZ

ANTONIO LAGUNA CERDA

MARTIN ROBERTO GAMEZ PASTRANA

MARIA ELENA GALINDO TOVAR

LOURDES GEORGINA IGLESIAS ANDREU

NANCY SANTANA BUZZY

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Materias

ARTIFICIAL SEED - (AUTORES) ORCHIDACEAE - (AUTORES) SOMATIC EMBRYOGENESIS - (AUTORES) BIOLOGÍA Y QUÍMICA - (CTI)

Resumen o descripción

Somatic embryoids obtained from germinated seeds in an MS culture media supplemented with naphthalene acetic acid (NAA), 6, bencylaminopurine (BAP) and indole-3-acetic acid (IAA), 2 mg·liter-1each, were encapsulated in sodium alginate in complex with calcium chloride (CaCl 2·2H2O) 75 mM matrix, to produce synthetic seeds, as a rescue and conservation strategy. In order to determinate the effect of physical and chemical factors, such as stored temperature and time, sodium alginate consistency, and mineral nutriments an plant growth regulators concentration on percentage and time for germination, and the viability of the synthetic seeds, different concentrations of: sodium alginate (2.0, 3.0 and 4.0 %), MS salts (37.5, 50 and 100 %) and BAP (0.5, 1.0 and 2.0 mg·liter -1) added to the encapsulation matrix, were tested at different temperatures (4, 20 and 25 °C) and incubation times (15 and 30 days). A hundred percent germination of synthetic seeds was obtained when 3 % sodium alginate in complex with CaCl2·2H2O at 75 mM was used; MS salts at 100 and 50 % and 2.0 mg·liter-1 BAP supplemented to the encapsulation matrix, achieved 64.54 y 62.77 % germination rate, respectively. No meaningful difference was observed between concentrations.

Fecha de publicación

2009

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://cicy.repositorioinstitucional.mx/jspui/handle/1003/782

Formato

application/pdf

Fuente

Revista Chapingo, Serie Horticultura, 15(2 SPL), 33-40, 2009

Idioma

Inglés

Sugerencia de citación

Lee-Espinosa, H. E., Murguía-González, J., Laguna-Cerda, A., García-Rosas, B., Gámez-Pastrana, M. R., Galindo-Tovar, M. E., . . . Santana-Buzzy, N. (2009). Somatic embryoids encapsulation of laelia anceps ssp. dawsonii to produce synthetic seed. [Encapsulación de embriones somáticos de Laelia anceps ssp. dawsonii para la producción de semilla sintética] Revista Chapingo, Serie Horticultura, 15(2 SPL), 33-40.

Repositorio Orígen

Repositorio Institucional CICY

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