Título

Laccase gene expression and vinasse biodegradation by Trametes hirsuta strain Bm-2

Autor

RAUL TAPIA TUSSELL

DAISY DE LA CARIDAD PEREZ BRITO

CLAUDIA GUADALUPE TORRES CALZADA

ALBERTO CORTES VELAZQUEZ

LILIANA MARIA ALZATE GAVIRIA

RUBI DEL ROSARIO CHABLE VILLACIS

SARA ELENA SOLIS PEREIRA

Nivel de Acceso

Acceso Abierto

Referencia de datos

datasetDOI/doi:10.3390/molecules200815147

Resumen o descripción

Vinasse is the dark-colored wastewater that is generated by bioethanol distilleries from feedstock molasses. The vinasse that is generated from molasses contains high amounts of pollutants, including phenolic compounds and melanoindin. The goal of this work was to study the expression of laccase genes in the Trametes hirsuta strain Bm-2, isolated in Yucatan, Mexico, in the presence of phenolic compounds, as well as its effectiveness in removing colorants from vinasse. In the presence of all phenolic compounds tested (guaiacol, ferulic acid, and vanillic acid), increased levels of laccase-encoding mRNA were observed. Transcript levels in the presence of guaiacol were 40 times higher than those in the control. The lcc1 and lcc2 genes of T. hirsuta were differentially expressed; guaiacol and vanillin induced the expression of both genes, whereas ferulic acid only induced the expression of lcc2. The discoloration of vinasse was concomitant with the increase in laccase activity. The highest value of enzyme activity (2543.7 U/mL) was obtained in 10% (v/v) vinasse, which corresponded to a 69.2% increase in discoloration. This study demonstrates the potential of the Bm-2 strain of T. hirsuta for the biodegradation of vinasse.

Fecha de publicación

1 de agosto de 2015

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Formato

application/pdf

Fuente

Molecules. 20(8), 15147-15157, 2015

Idioma

Inglés

Relación

&

Solís-Pereira, S. (2015). Laccase Gene Expression and Vinasse Biodegradation by Trametes hirsuta Strain Bm-2. Molecules, 20(8), 15147-15157.

Sugerencia de citación

Tapia-Tussell, R., Pérez-Brito, D., Torres-Calzada, C., Cortés-Velázquez, A., Alzate-Gaviria, L., Chablé-Villacís, R.,

Repositorio Orígen

Repositorio Institucional CICY

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2

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