Título
Genome-wide association mapping for resistance to leaf rust, stripe rust and tan spot in wheat reveals potential candidate genes
Autor
Philomin Juliana
Ravi Singh
Jesse Poland
Gary Bergstrom
JULIO HUERTA-ESPINO
sridhar bhavani
Jose Crossa
Mark Sorrells
Nivel de Acceso
Acceso Abierto
Materias
Rusts - (AGROVOC) Wheats - (AGROVOC) Disease resistance - (AGROVOC) Genome-Wide Association Studies - (AGROVOC) Leaf Rust - (AGROVOC) Stripe Rust - (AGROVOC) Tan Spot - (AGROVOC) WHEAT - (AGROVOC) INFECTIOUS DISEASES - (AGROVOC) DISEASE RESISTANCE - (AGROVOC) CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA - (CTI)
Resumen o descripción
Leaf rust (LR), stripe rust (YR) and tan spot (TS) are some of the important foliar diseases in wheat (Triticum aestivum L.). To identify candidate resistance genes for these diseases in CIMMYT?s (International Maize and Wheat Improvement Center) International bread wheat screening nurseries, we used genome-wide association studies (GWAS) in conjunction with information from the population sequencing map and Ensembl plants. Wheat entries were genotyped using genotyping-by-sequencing and phenotyped in replicated trials. Using a mixed linear model, we observed that seedling resistance to LR was associated with 12 markers on chromosomes 1DS, 2AS, 2BL, 3B, 4AL, 6AS and 6AL, and seedling resistance to TS was associated with 14 markers on chromosomes 1AS, 2AL, 2BL, 3AS, 3AL, 3B, 6AS and 6AL. Seedling and adult plant resistance (APR) to YR were associated with several markers at the distal end of chromosome 2AS. In addition, YR APR was also associated with markers on chromosomes 2DL, 3B and 7DS. The potential candidate genes for these diseases included several resistance genes, receptor-like serine/threonine-protein kinases and defense-related enzymes. However, extensive LD in wheat that decays at about 5 × 107 bps, poses a huge challenge for delineating candidate gene intervals and candidates should be further mapped, functionally characterized and validated. We also explored a segment on chromosome 2AS associated with multiple disease resistance and identified seventeen disease resistance linked genes. We conclude that identifying candidate genes linked to significant markers in GWAS is feasible in wheat, thus creating opportunities for accelerating molecular breeding.
Fecha de publicación
2018
Tipo de publicación
Artículo
Recurso de información
Formato
application/pdf
Idioma
Inglés
Audiencia
Investigadores
Repositorio Orígen
Repositorio Institucional de Publicaciones Multimedia del CIMMYT
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