Título
Functional polymorphism for crtRB1 gene loci in tropical maize (Zea mays L.) inbred lines
Autor
Pervez Zaidi
Prakash Kuchanur
Nivel de Acceso
Acceso Abierto
Materias
Resumen o descripción
Maize (Zea mays L.) is an important cropknown for its carotenoid diversity amongcereals which accumulates significant levels of proA (provitamin A) and non-proA carotenoids itskernels. The proA components of maize endosperm promises to solve themajor global problem VAD(Vitamin A deficiency). Among several genes involved in-carotene biosynthetic pathway, crtRB1isvery important gene associated with three polymorphismsviz., 5 'TE, In Del4 and 3'TE (TransposableElement) responsible for variation in carotenoid levels in maize endosperm. Due to insertion of TE at 3'UTR (Un Translated Region), crtRB1again exhibits polymorphism with 3 alleles, however, only allele1(favourable allele; 543bp amplicon) of this crtRB1-3'TE gene will double the-carotene concentrationin maize endosperm and allele 2 and 3 termed as unfavourable. This study was undertaken to find outthe allelic difference for crtRB1gene loci. Totally 228 tropical maize inbred lines were screened for allele1of crtRB1gene using crtRB1-3’TE gene specific markers. Among 228 in breds, 226 inbreds showed thepresence of allele 2 and the two inbredsVL1016247 and VL1016213 possessed both alleles 1 and 2 whichare found to be heterozygous for crtRB1 loci. This study indicated the possible use of (VL1016247 andVL1016213) these two inbreds for developing provitaminA (proA) rich maize hybrids using markerassisted selection (MAS).
Fecha de publicación
2017
Tipo de publicación
Artículo
Recurso de información
Formato
application/pdf
Idioma
Inglés
Audiencia
Investigadores
Repositorio Orígen
Repositorio Institucional de Publicaciones Multimedia del CIMMYT
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