Título
Development of a biosensor based on a-SixC1-x:H to detect enterotoxigenic Escherichia coli
Autor
JOSE LUIS HERRERA CELIS
Colaborador
CLAUDIA REYES BETANZO (Asesor de tesis)
ABDU ORDUÑA DIAZ (Asesor de tesis)
Nivel de Acceso
Acceso Abierto
Materias
Amorphous silicon carbide - (CARBURO DE SILICIO AMORFO) Biosensor - (BIOSENSOR) Enterotoxigenic escherichia coli - (ENTEROTOXIGENIC ESCHERICHIA COLI) Characterization - (CARACTERIZACIÓN) Biofunctionalization - (BIOFUNCIONALIZACIÓN) CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA - (CTI) FÍSICA - (CTI) ELECTRÓNICA - (CTI)
Resumen o descripción
The food industry and clinical analysis, among other sectors, require the development of techniques and devices that detect pathogens, while the development of implantable devices needs biocompatible materials with low degradation in biological environment to increase the lifetime of the device. Throughout this work, hydrogenated amorphous silicon-carbon (a-SixC1-x:H) alloy is proposed, obtained, characterized and incorporated into the development of a proposed interdigitated microelectrode array (PIMA) to capture the bacteria of enterotoxigenic Escherichia coli (E. coli, ETEC). a-SixC1-x:H is obtained by the technique of plasma-enhanced chemical vapor deposition (PECVD) using methane and silane as precursor gases under high hydrogen dilution and low power density in order to improve its biocompatibility. Furthermore, considering the projection of the material in biosensors and the effect of the precursor gas ratio on the material properties, films with different precursor gas ratio were deposited and characterized. The result was a set of films with low density of CHn groups, which was also subject under studied.
Simultaneously, the PIMA was designed and simulated using CoventorWare® software. Structurally the PIMA incorporates two layers of a-SixC1-x:H, one between and another on microelectrode, on which a biofunctionalization layer is formed. The aim of this layer is to capture the bacteria by affinity interactions. Functionally the PIMA is a transducer based on electrical impedance, namely the capture of E. coli bacteria causes changes in the electrical properties of the medium between and on the microelectrodes of the array, which are associated with changes in electrical impedance. The simulations were made with the purpose of knowing the operation that the PIMA would have under operating conditions (with bacterial environment) and of analyzing the design aspects that could affect or increase the sensitivity of the array. One of the results of the simulations was that the conductivity of the layer of a-SixC1-x:H on microelectrodes should be as high as possible to reduce its effect on the sensitivity of the biosensor, therefore diborane as doping gas was incorporated during the depositions of some films, which were also characterized.
Editor
Instituto Nacional de Astrofísica, Óptica y Electrónica
Fecha de publicación
noviembre de 2016
Tipo de publicación
Tesis de doctorado
Versión de la publicación
Versión aceptada
Recurso de información
Formato
application/pdf
Idioma
Inglés
Audiencia
Estudiantes
Investigadores
Público en general
Sugerencia de citación
Herrera-Celis J.L.
Repositorio Orígen
Repositorio Institucional del INAOE
Descargas
3620