Título

Subtelomeric silencing of the MTL3 locus of Candida glabrata requires yKu70, yKu80, and Rif1 proteins

Autor

CANDY YURIRIA RAMIREZ ZAVALETA

GRISELDA EDITH SALAS DELGADO

ALEJANDRO DE LAS PEÑAS NAVA

IRENE BEATRIZ CASTAÑO NAVARRO

Nivel de Acceso

En Embargo

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Identificador alterno

doi: https://dx.doi.org/10.1128%2FEC.00129-10

Materias

BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) BIOLOGÍA ANIMAL (ZOOLOGÍA) - (CTI) MICROBIOLOGÍA - (CTI) MICROBIOLOGÍA - (CTI)

Resumen o descripción

"Candida glabrata is a haploid opportunistic fungal pathogen that is phylogenetically related to Saccharomyces cerevisiae. Even though C. glabrata has no known sexual cycle, it contains, like S. cerevisiae, three mating type-like loci (MTL) called MTL1, MTL2, and MTL3, as well as most of the genes required for mating, meiosis, and sporulation. MTL1 is localized at an internal position on chromosome B and is thought to be the locus corresponding to the MAT locus in S. cerevisiae. MTL2 and MTL3 are localized close to two telomeres on different chromosomes (29.4 kb from Chr E-L and 10.5 kb from Chr B-L, respectively). By using URA3 reporter gene insertions at the three MTL loci, we found that in contrast to the case for S. cerevisiae, only MTL3 is subject to transcriptional silencing while MTL2 is transcriptionally active, and this is in agreement with previously reported data. We found that the silencing of MTL3 is nucleated primarily at the left telomere of chromosome B and spreads over 12 kb to MTL3, rather than nucleating at flanking, closely positioned cis-acting silencers, like those flanking HMR and HML of S. cerevisiae. Interestingly, the silencing of MTL3 absolutely requires the yKu70, yKu80, and Rif1 proteins, in sharp contrast to the silencing of the HM loci of S. cerevisiae. In addition, we found that several cell type-specific genes are expressed in C. glabrata regardless of the presence, or even absence, of mating type information at any of the MTL loci."

Editor

American Society for Microbiology

Fecha de publicación

octubre de 2010

Tipo de publicación

Artículo

Recurso de información

http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1149

Formato

application/pdf

Idioma

Inglés

Relación

&

Castaño, I. (2010). Subtelomeric Silencing of the MTL3 Locus of Candida glabrata Requires yKu70, yKu80, and Rif1 Proteins . Eukaryotic Cell, 9(10), 1602–1611.

Audiencia

Público en general

Sugerencia de citación

Ramírez-Zavaleta, C. Y., Salas-Delgado, G. E., De Las Peñas, A.,

Repositorio Orígen

Repositorio IPICYT

Descargas

190