Título
Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance
Autor
MARIA AZUCENA ORTEGA AMARO
AIDA ARACELI RODRIGUEZ HERNANDEZ
MARGARITA RODRIGUEZ Y DOMINGUEZ KESSLER
ELOISA HERNANDEZ LUCERO
SERGIO ROSALES MENDOZA
ALEJANDRO IBAÑEZ SALAZAR
PABLO DELGADO SANCHEZ
JUAN FRANCISCO JIMENEZ BREMONT
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: https://doi.org/10.3389/fpls.2014.00782
Materias
Resumen o descripción
"Proteins with glycine-rich signatures have been reported in a wide variety of organisms including plants, mammalians, fungi, and bacteria. Plant glycine-rich protein genes exhibit developmental and tissue-specific expression patterns. Herein, we present the characterization of the AtGRDP2 gene using Arabidopsis null and knockdown mutants and, Arabidopsis and lettuce over-expression lines. AtGRDP2 encodes a short glycine-rich domain protein, containing a DUF1399 domain and a putative RNA recognition motif (RRM). AtGRDP2 transcript is mainly expressed in Arabidopsis floral organs, and its deregulation in Arabidopsis Atgrdp2 mutants and 35S::AtGRDP2 over-expression lines produces alterations in development. The 35S::AtGRDP2 over-expression lines grow faster than the WT, while the Atgrdp2 mutants have a delay in growth and development. The over-expression lines accumulate higher levels of indole-3-acetic acid and, have alterations in the expression pattern of ARF6, ARF8, and miR167 regulators of floral development and auxin signaling. Under salt stress conditions, 35S::AtGRDP2 over-expression lines displayed higher tolerance and increased expression of stress marker genes. Likewise, transgenic lettuce plants over-expressing the AtGRDP2 gene manifest increased growth rate and early flowering time. Our data reveal an important role for AtGRDP2 in Arabidopsis development and stress response, and suggest a connection between AtGRDP2 and auxin signaling."
Editor
Frontiers Research Foundation
Fecha de publicación
enero de 2015
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Idioma
Inglés
Audiencia
Público en general
Sugerencia de citación
Ortega-Amaro MA, Rodríguez-Hernández AA, Rodríguez-Kessler M, Hernández-Lucero E, Rosales-Mendoza S, Ibáñez-Salazar A, Delgado-Sánchez P and Jiménez-Bremont JF (2015) Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance. Front.Plant Sci. 5:782. doi: 10.3389/fpls.2014.00782
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Repositorio IPICYT
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