Título

Monitoring lipase/esterase activity by stopped flow in a sequential injection analysis system using p-nitrophenyl butyrate

Autor

JORGE ENRIQUE PLIEGO SANDOVAL

JUAN CARLOS MATEOS DIAZ

JORGE ALBERTO RODRIGUEZ GONZALEZ

Francisco Valero

Maria del Mar Baeza Labat

ALEJANDRO RICARDO FEMAT FLORES

ROSA MARIA CAMACHO RUIZ

GEORGINA CORAL SANDOVAL FABIAN

Enrique J Herrera López

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Identificador alterno

doi: http://dx.doi.org/10.3390/s150202798

Materias

Monitoring - (AUTOR) Lipase/esterase activity - (LIPASE) Sequential injection analysis; - (AUTOR) Stopped flow - (AUTOR) P-nitrophenyl esters - (AUTOR) BIOLOGÍA Y QUÍMICA - (CTI) QUÍMICA - (CTI) QUÍMICA FÍSICA - (CTI) ELECTROQUÍMICA - (CTI)

Resumen o descripción

"Lipases and esterases are biocatalysts used at the laboratory and industrial level. To obtain the maximum yield in a bioprocess, it is important to measure key variables, such as enzymatic activity. The conventional method for monitoring hydrolytic activity is to take out a sample from the bioreactor to be analyzed off-line at the laboratory. The disadvantage of this approach is the long time required to recover the information from the process, hindering the possibility to develop control systems. New strategies to monitor lipase/esterase activity are necessary. In this context and in the first approach, we proposed a lab-made sequential injection analysis system to analyze off-line samples from shake flasks. Lipase/esterase activity was determined using p-nitrophenyl butyrate as the substrate. The sequential injection analysis allowed us to measure the hydrolytic activity from a sample without dilution in a linear range from 0.05-1.60 U/mL, with the capability to reach sample dilutions up to 1000 times, a sampling frequency of five samples/h, with a kinetic reaction of 5 min and a relative standard deviation of 8.75%. The results are promising to monitor lipase/esterase activity in real time, in which optimization and control strategies can be designed."

Editor

MDPI AG

Fecha de publicación

2015

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1290

http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1343

Formato

application/pdf

Idioma

Inglés

Audiencia

Público en general

Sugerencia de citación

Pliego, J.; Mateos, J.C.; Rodriguez, J.; Valero, F.; Baeza, M.; Femat, R.; Camacho, R.; Sandoval, G.; Herrera-López, E.J. Monitoring Lipase/Esterase Activity by Stopped Flow in a Sequential Injection Analysis System Using p-Nitrophenyl Butyrate. Sensors 2015, 15, 2798-2811.

Repositorio Orígen

Repositorio IPICYT

Descargas

582