Título

2D-DIGE as a strategy to identify serum biomarkers in Mexican patients with Type-2 diabetes with different body mass index

Autor

Erik Elvin Gómez Cardona

Eric Edmundo Hernández Domínguez

AIDA JIMENA VELARDE SALCEDO

Alberto Barrera Pacheco

Agustín Diaz Gois

Antonio de León Rodríguez

Ana Paulina Barba de la Rosa

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Identificador alterno

doi: https://doi.org/10.1038/srep46536

Materias

Predictive markers - (AUTOR) Protein–protein interaction networks - (AUTOR) INGENIERÍA Y TECNOLOGÍA - (CTI) CIENCIAS TECNOLÓGICAS - (CTI) CIENCIAS TECNOLÓGICAS - (CTI)

Resumen o descripción

"Obesity and type 2 diabetes (T2D) are the most prevalent and serious metabolic diseases affecting people worldwide. However racial and ethnic disparities seems to be a risk factor for their development. Mexico has been named as one of the largest populations with the highest prevalence of diabetes and obesity. The aim of this study was to identify novel T2D-associated proteins in Mexican patients. Blood samples were collected from 62 Mexican patients with T2D and they were grouped according to their body mass index (BMI). A panel of 10 diabetes and obesity serum markers was determined using MAGPIX. A comparative proteomics study was performed using two-dimensional difference in-gel electrophoresis (2D-DIGE) followed by mass spectrometry (LC-MS/MS). We detected 113 spots differentially accumulated, in which 64 unique proteins were identified, proteins that were involved in metabolism pathways, molecular transport, and cellular signalling. Four proteins (14-3-3, ApoH, ZAG, and OTO3) showing diabetes-related variation and also changes in relation to obesity were selected for further validation by western blotting. Our results reveal new diabetes related proteins present in the Mexican population. These could provide additional insight into the understanding of diabetes development in Mexican population and may also be useful candidate biomarkers."

Editor

Nature Publishing Group

Fecha de publicación

abril de 2017

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1440

Formato

application/pdf

Relación

&

Barba de la Rosa, A. P. (2017). 2D-DIGE as a strategy to identify serum biomarkers in Mexican patients with Type-2 diabetes with different body mass index. Scientific Reports, 7, 46536. http://doi.org/10.1038/srep46536

Sugerencia de citación

Gómez-Cardona, E. E., Hernández-Domínguez, E. E., Velarde-Salcedo, A. J., Pacheco, A.-B.-, Diaz-Gois, A., De León-Rodríguez, A.,

Repositorio Orígen

Repositorio IPICYT

Descargas

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