Título

Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica

Autor

SERGIO ANGEL GARCIA ECHAURI

Manuel Gidekel

Ana Gutiérrez Moraga

LEANDRO GABRIEL ORDOÑEZ ACEVEDO

JUAN ANTONIO ROJAS CONTRERAS

Ana Paulina Barba de la Rosa

Antonio de León Rodríguez

Nivel de Acceso

Acceso Abierto

Identificador alterno

doi: https://doi.org/10.1016/j.procbio.2009.04.021

Resumen o descripción

"Superoxide dismutase (SOD) catalyzes the conversion of the superoxide radical (O2−) into oxygen and hydrogen peroxide. Deschampsia antarctica is a plant that grows in Antarctica and survives to extreme low temperature and high UV radiation, thus it is an ideal model to study novel antioxidants. A cDNA Cu/Zn-SOD gene from D. antarctica was cloned into a pET vector and expressed in Escherichia coli BL21-SI. 112 mg/L of recombinant Cu/Zn-SOD was attained in batch cultures in bioreactor. Using Ni-affinity gel chromatography, the recombinant Cu/Zn-SOD was recovered with a purity of 90% and a specific enzyme activity of 749 at 25 °C. However, zymogram test showed that the enzyme has more activity at 4 °C. This D. antarctica SOD could be used to reduce the oxidation of refrigerated and frozen foods."

Editor

Elsevier Science LTD

Fecha de publicación

septiembre de 2009

Tipo de publicación

Artículo

Versión de la publicación

Versión aceptada

Formato

application/pdf

Sugerencia de citación

Sergio A. García Echauri, Manuel Gidekel, Ana Gutiérrez Moraga, Leandro G. Ordóñez, Juan A. Rojas Contreras, Ana P. Barba de la Rosa, Antonio De León Rodríguez, Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica, Process Biochemistry, Volume 44, Issue 9, 2009, Pages 969-974.

Repositorio Orígen

Repositorio IPICYT

Descargas

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