Título
Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica
Autor
SERGIO ANGEL GARCIA ECHAURI
Manuel Gidekel
Ana Gutiérrez Moraga
LEANDRO GABRIEL ORDOÑEZ ACEVEDO
JUAN ANTONIO ROJAS CONTRERAS
Ana Paulina Barba de la Rosa
Antonio de León Rodríguez
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: https://doi.org/10.1016/j.procbio.2009.04.021
Materias
Resumen o descripción
"Superoxide dismutase (SOD) catalyzes the conversion of the superoxide radical (O2−) into oxygen and hydrogen peroxide. Deschampsia antarctica is a plant that grows in Antarctica and survives to extreme low temperature and high UV radiation, thus it is an ideal model to study novel antioxidants. A cDNA Cu/Zn-SOD gene from D. antarctica was cloned into a pET vector and expressed in Escherichia coli BL21-SI. 112 mg/L of recombinant Cu/Zn-SOD was attained in batch cultures in bioreactor. Using Ni-affinity gel chromatography, the recombinant Cu/Zn-SOD was recovered with a purity of 90% and a specific enzyme activity of 749 at 25 °C. However, zymogram test showed that the enzyme has more activity at 4 °C. This D. antarctica SOD could be used to reduce the oxidation of refrigerated and frozen foods."
Editor
Elsevier Science LTD
Fecha de publicación
septiembre de 2009
Tipo de publicación
Artículo
Versión de la publicación
Versión aceptada
Recurso de información
Formato
application/pdf
Sugerencia de citación
Sergio A. García Echauri, Manuel Gidekel, Ana Gutiérrez Moraga, Leandro G. Ordóñez, Juan A. Rojas Contreras, Ana P. Barba de la Rosa, Antonio De León Rodríguez, Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica, Process Biochemistry, Volume 44, Issue 9, 2009, Pages 969-974.
Repositorio Orígen
Repositorio IPICYT
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