Título

Expansion of human hematopoietic cells from umbilical cord blood using roller bottles in CO2 and CO2-free atmosphere

Autor

Hera Andrade Zaldívar

Marco Antonio Kalixto Sánchez

Ana Paulina Barba de la Rosa

Antonio de León Rodríguez

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Identificador alterno

doi: https://doi.org/10.1089/scd.2010.0236

Materias

BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) BIOLOGÍA CELULAR - (CTI) BIOLOGÍA CELULAR - (CTI)

Resumen o descripción

"In this work, we evaluated the expansion of human hematopoietic stem cells from umbilical cord blood in roller bottles. The Iscove's modified Dulbecco's medium, the Stem Pro 34-SFM medium, and the L-15 Leibovitz's medium for cultures in CO2-free atmosphere were assessed. At day 5 of culture, total colony forming unit expansions of 14.44 ± 3.74, 11.20 ± 6.37, and 17.25 ± 3.65-folds were attained, respectively. The expansion reached using L-15 medium in roller bottles was around 10 times higher than that achieved in the static control cultures. To our knowledge, this is the first report of cultures in CO2-free atmosphere to expand cord blood human hematopoietic stem cells and it opens a new branch of possibilities for culturing and clinical applications."

Editor

Mary Ann Liebert, Inc

Fecha de publicación

abril de 2011

Tipo de publicación

Artículo

Versión de la publicación

Versión aceptada

Recurso de información

http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1487

Formato

application/pdf

Sugerencia de citación

Hera Andrade-Zaldívar, Marco A. Kalixto-Sánchez, Ana P. Barba de la Rosa, and Antonio De León-Rodríguez.Stem Cells and Development.Apr 2011.ahead of printhttp://doi.org/10.1089/scd.2010.0236

Repositorio Orígen

Repositorio IPICYT

Descargas

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