Título

Histone deacetylase HDA-2 regulates Trichoderma atroviride growth, conidiation, blue light perception, and oxidative stress responses

Autor

MACARIO OSORIO CONCEPCION

GEMA ROSA CRISTOBAL MONDRAGON

BRAULIO GUTIERREZ MEDINA

J SERGIO CASAS FLORES

Nivel de Acceso

Acceso Abierto

Identificador alterno

doi: http://doi.org/10.1128/AEM.02922-16

Resumen o descripción

"Fungal blue-light photoreceptors have been proposed as integrators of light and oxidative stress. However, additional elements participating in the integrative pathway remain to be identified. In Trichoderma atroviride, the blue-light regulator (BLR) proteins BLR-1 and -2 are known to regulate gene transcription, mycelial growth, and asexual development upon illumination, and recent global transcriptional analysis revealed that the histone deacetylase-encoding gene hda-2 is induced by light. Here, by assessing responses to stimuli in wild-type and Δhda-2 backgrounds, we evaluate the role of HDA-2 in the regulation of genes responsive to light and oxidative stress. Δhda-2 strains present reduced growth, misregulation of the con-1 gene, and absence of conidia in response to light and mechanical injury. We found that the expression of hda-2 is BLR-1 dependent and HDA-2 in turn is essential for the transcription of early and late light-responsive genes that include blr-1, indicating a regulatory feedback loop. When subjected to reactive oxygen species (ROS), Δhda-2 mutants display high sensitivity whereas Δblr strains exhibit the opposite phenotype. Consistently, in the presence of ROS, ROS-related genes show high transcription levels in wild-type and Δblr strains but misregulation in Δhda-2 mutants. Finally, chromatin immunoprecipitations of histone H3 acetylated at Lys9/Lys14 on cat-3 and gst-1 promoters display low accumulation of H3K9K14ac in Δblr and Δhda-2 strains, suggesting indirect regulation of ROS-related genes by HDA-2. Our results point to a mutual dependence between HDA-2 and BLR proteins and reveal the role of these proteins in an intricate gene regulation landscape in response to blue light and ROS."

Editor

American Society for Microbiology

Fecha de publicación

febrero de 2017

Tipo de publicación

Artículo

Versión de la publicación

Versión aceptada

Formato

application/pdf

Relación

&

Casas-Flores, S. (2017). Histone Deacetylase HDA-2 Regulates Trichoderma atroviride Growth, Conidiation, Blue Light Perception, and Oxidative Stress Responses. Applied and Environmental Microbiology, 83(3), e02922–16. http://doi.org/10.1128/AEM.02922-16

Sugerencia de citación

Osorio-Concepción, M., Cristóbal-Mondragón, G. R., Gutiérrez-Medina, B.,

Repositorio Orígen

Repositorio IPICYT

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