Título
Histone deacetylase HDA-2 regulates Trichoderma atroviride growth, conidiation, blue light perception, and oxidative stress responses
Autor
MACARIO OSORIO CONCEPCION
GEMA ROSA CRISTOBAL MONDRAGON
BRAULIO GUTIERREZ MEDINA
J SERGIO CASAS FLORES
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: http://doi.org/10.1128/AEM.02922-16
Materias
Resumen o descripción
"Fungal blue-light photoreceptors have been proposed as integrators of light and oxidative stress. However, additional elements participating in the integrative pathway remain to be identified. In Trichoderma atroviride, the blue-light regulator (BLR) proteins BLR-1 and -2 are known to regulate gene transcription, mycelial growth, and asexual development upon illumination, and recent global transcriptional analysis revealed that the histone deacetylase-encoding gene hda-2 is induced by light. Here, by assessing responses to stimuli in wild-type and Δhda-2 backgrounds, we evaluate the role of HDA-2 in the regulation of genes responsive to light and oxidative stress. Δhda-2 strains present reduced growth, misregulation of the con-1 gene, and absence of conidia in response to light and mechanical injury. We found that the expression of hda-2 is BLR-1 dependent and HDA-2 in turn is essential for the transcription of early and late light-responsive genes that include blr-1, indicating a regulatory feedback loop. When subjected to reactive oxygen species (ROS), Δhda-2 mutants display high sensitivity whereas Δblr strains exhibit the opposite phenotype. Consistently, in the presence of ROS, ROS-related genes show high transcription levels in wild-type and Δblr strains but misregulation in Δhda-2 mutants. Finally, chromatin immunoprecipitations of histone H3 acetylated at Lys9/Lys14 on cat-3 and gst-1 promoters display low accumulation of H3K9K14ac in Δblr and Δhda-2 strains, suggesting indirect regulation of ROS-related genes by HDA-2. Our results point to a mutual dependence between HDA-2 and BLR proteins and reveal the role of these proteins in an intricate gene regulation landscape in response to blue light and ROS."
Editor
American Society for Microbiology
Fecha de publicación
febrero de 2017
Tipo de publicación
Artículo
Versión de la publicación
Versión aceptada
Recurso de información
Formato
application/pdf
Relación
&
Casas-Flores, S. (2017). Histone Deacetylase HDA-2 Regulates Trichoderma atroviride Growth, Conidiation, Blue Light Perception, and Oxidative Stress Responses. Applied and Environmental Microbiology, 83(3), e02922–16. http://doi.org/10.1128/AEM.02922-16
Sugerencia de citación
Osorio-Concepción, M., Cristóbal-Mondragón, G. R., Gutiérrez-Medina, B.,
Repositorio Orígen
Repositorio IPICYT
Descargas
470