Título
Effect of graphene oxide on bacteria and peripheral blood mononuclear cells
Autor
Jessica Rosaura Campos Delgado
José Gil Munguía López
Benjamin Fragneaud
Joyce Araujo
Francisco Gonzalez
HUGO RICARDO NAVARRO CONTRERAS
IVAN NELINHO PEREZ MALDONADO
Antonio de León Rodríguez
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: https://doi.org/10.5301/jabfm.5000325
Materias
Resumen o descripción
"Background Driven by the potential biological applications of graphene, many groups have studied the response of cells exposed to graphene oxide (GO). In particular, investigations of bacteria indicate that there are 2 crucial parameters, which so far have only been investigated separately: GO size and exposure methodology. Our study took into account both parameters. We carefully characterized the samples to catalog sizes and structural properties, and tested different exposure methodologies: exposure in saline solution and in the presence of growth media. Furthermore, we performed experiments with peripheral blood mononuclear cells exposed to our GO materials. Methods Atomic force microscopy, scanning electron microscopy, Raman spectroscopy, X-ray photoelectron spectroscopy and transmission electron microscopy were used to characterize the morphology and composition of different samples of GO: GO-H2O, GO-PBS and GO-MG. Our samples had 2D sizes of ?100 nm (GO-H2O and GO-PBS) and >2 µm (GO-MG). We tested antibacterial activity and cytotoxicity toward peripheral blood mononuclear cells of 3 different GO samples. Results A size-dependent growth inhibition of Escherichia coli (DH5 ?) in suspension was found, which proved that this effect depends strongly on the protocol followed for exposure. Hemocompatibility was confirmed by exposing peripheral blood mononuclear cells to materials for 24 hours; viability and apoptosis tests were also carried out. Conclusions Our experiments provide vital information for future applications of GO in suspension. If its antibacterial properties are to be potentiated, care should be taken to select 2D sizes in the micrometer range, and exposure should not be carried out in the presence of grow media."
Editor
Wichtig Publishing
Fecha de publicación
2016
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Relación
&
Functional Materials, 14(4), 423–430.
Sugerencia de citación
Campos-Delgado, J., Castro, K. L. S., Munguia-Lopez, J. G., González, A. K., Mendoza, M. E., Fragneaud, B., … Achete, C. A. (2016). Effect of Graphene Oxide on Bacteria and Peripheral Blood Mononuclear Cells. Journal of Applied Biomaterials
Repositorio Orígen
Repositorio IPICYT
Descargas
258