Título

Evaluation of the expression and function of the P2X7 receptor and ART1 in human regulatory T-cell subsets

Autor

JUAN DIEGO CORTES GARCIA

CINTYA DEL REFUGIO LOPEZ LOPEZ

NANCY CORTEZ ESPINOSA

MARIANA HAYDEE GARCIA HERNANDEZ

JUAN MANUEL GUZMAN FLORES

ESTHER LAYSECA ESPINOSA

LILIANA PORTALES CERVANTES

DIANA PATRICIA PORTALES PEREZ

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Identificador alterno

doi: https://doi.org/10.1016/j.imbio.2015.07.018

Materias

P2X7 receptor - (AUTOR) ART1 - (AUTOR) ATP - (AUTOR) NAD - (AUTOR) Immune regulation - (AUTOR) CD39 - (AUTOR) BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) BIOLOGÍA MOLECULAR - (CTI) BIOLOGÍA MOLECULAR - (CTI)

Resumen o descripción

"Regulatory T cells that express CD39 (CD39+ Treg) exhibit specific immunomodulatory properties. Ectonucleotidase CD39 hydrolyses ATP and ADP. ATP is a ligand of the P2X7 receptor and induces the shedding of CD62L and apoptosis. However, the role of ATP in CD39+ Treg cells has not been defined. Furthermore, NAD can activate the P2X7 receptor via ADP-ribosyltransferase (ART) enzymes and cause cell depletion in murine models. We evaluated the expression and function of P2X7 and ART1 in CD39+ Treg and CD39- Treg cells in the presence or absence of ATP and NAD. We isolated peripheral blood mononuclear cells from healthy subjects and purified CD4+ T cells, CD4+ CD25+ T cells and CD4+ CD25+ CD39+ T cells. P2X7 and ART1 expression was assessed by flow cytometry and real-time PCR. Our results showed low P2X7 expression on CD39+ Treg cells and higher levels of ART1 expression in CD4+ CD39+ T cells than the other subtypes studied. Neither shedding of CD62L nor cell death of CD39+ Treg or CD39- Treg cells was observed by 1mM ATP or 60 mu M NAD. In contrast, P2Xs receptor-dependent proliferation with 300 p,M ATP, was inhibited by NAD in the different cell types analysed. The NAD proliferation-inhibition was increased with P2X5 and A2a agonist and was reversed with P2X5 and A2a antagonist, therefore NAD inhibits P2Xs-dependent proliferation and A2a activation. In conclusion, our results suggest that the altered function and expression of P2X7 and ART1 in the human CD39+ Treg or CD39 Treg cells could participate in the resistance against cell death induced by ATP or NAD. (C) 2015 Elsevier GmbH. All rights reserved."

Editor

Elsevier

Fecha de publicación

2016

Tipo de publicación

Artículo

Versión de la publicación

Versión aceptada

Recurso de información

http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1945

Formato

application/pdf

Sugerencia de citación

Juan D. Cortés-Garcia, Cintya López-López, Nancy Cortez-Espinosa, Mariana H. García-Hernández, Juan M. Guzmán-Flores, Esther Layseca-Espinosa, Liliana Portales-Cervantes, Diana P. Portales-Pérez, Evaluation of the expression and function of the P2X7 receptor and ART1 in human regulatory T-cell subsets, Immunobiology, Volume 221, Issue 1, 2016, Pages 84-93.

Repositorio Orígen

Repositorio IPICYT

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