Título

PEST sequences from a cactus dehydrin regulate its proteolytic degradation

Autor

ADRIANA LETICIA SALAZAR RETANA

Israel Maruri López

Itzell Eurídice Hernández Sánchez

ALICIA BECERRA FLORA

María de la Luz Guerrero González

JUAN FRANCISCO JIMENEZ BREMONT

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Identificador alterno

doi: https://doi.org/10.7717/peerj.6810

Materias

Dehydrin - (AUTOR) Intrinsically disordered proteins - (AUTOR) Pest sequences - (AUTOR) Protein degradation - (AUTOR) BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) BIOLOGÍA MOLECULAR - (CTI) BIOLOGÍA MOLECULAR - (CTI)

Resumen o descripción

"Dehydrins (DHNs) are intrinsically disordered proteins expressed under cellular dehydration-related stresses. In this study, we identified potential proteolytic PEST sequences located at the central and C-terminal regions from the Opuntia streptacantha OpsDHN1 protein. In order to evaluate these PEST sequences as proteolytic tags, we generated a translational fusion with the GUS reporter protein and OpsDHN1 coding sequence. We found a GUS degradation effect in tobacco agro-infiltrated leaves and Arabidopsis transgenic lines that expressed the fusion GUS::OpsDHN1 full-length. Also, two additional translational fusions between OpsDHN1 protein fragments that include the central (GUS::PEST-1) or the C-terminal (GUS::PEST-2) PEST sequences were able to decrease the GUS activity, with PEST-2 showing the greatest reduction in GUS activity. GUS signal was abated when the OpsDHN1 fragment that includes both PEST sequences (GUS::PEST-1-2) were fused to GUS. Treatment with the MG132 proteasome inhibitor attenuated the PEST-mediated GUS degradation. Point mutations of phosphorylatable residues in PEST sequences reestablished GUS signal, hence these sequences are important during protein degradation. Finally, in silico analysis identified potential PEST sequences in other plant DHNs. This is the first study reporting presence of PEST motifs in dehydrins."

Editor

PeerJ, Inc

Fecha de publicación

2019

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2140

Formato

application/pdf

Sugerencia de citación

Salazar-Retana AL, Maruri-López I, Hernández-Sánchez IE, Becerra-Flora A, Guerrero-González MdlL, Jiménez-Bremont JF. 2019. PEST sequences from a cactus dehydrin regulate its proteolytic degradation. PeerJ 7:e6810 https://doi.org/10.7717/peerj.6810

Repositorio Orígen

Repositorio IPICYT

Descargas

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