Título
Secretion of an argonaute protein by a parasitic nematode and the evolution of its siRNA guides
Autor
Wang-ngai Chow
Georgios Koutsovoulos
CESARE MOISES OVANDO VAZQUEZ
Kyriaki Neophytou
JOSE ROBERTO BERMUDEZ BARRIENTOS
Dominik Laetsch
Elaine Robertson
Sujai Kumar
Julie M Claycomb
Mark Blaxter
Cei Abreu_Goodger
Amy Buck
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: https://doi.org/10.1093/nar/gkz142
Materias
Resumen o descripción
"Extracellular RNA has been proposed to mediate communication between cells and organisms however relatively little is understood regarding how specific sequences are selected for export. Here, we describe a specific Argonaute protein (exWAGO) that is secreted in extracellular vesicles (EVs) released by the gastrointestinal nematode Heligmosomoides bakeri, at multiple copies per EV. Phylogenetic and gene expression analyses demonstrate exWAGO orthologues are highly conserved and abundantly expressed in related parasites but highly diverged in free-living genus Caenorhabditis. We show that the most abundant small RNAs released from the nematode parasite are not microRNAs as previously thought, but rather secondary small interfering RNAs (siRNAs) that are produced by RNA-dependent RNA Polymerases. The siRNAs that are released in EVs have distinct evolutionary properties compared to those resident in free-living or parasitic nematodes. Immunoprecipitation of exWAGO demonstrates that it specifically associates with siRNAs from transposons and newly evolved repetitive elements that are packaged in EVs and released into the host environment. Together this work demonstrates molecular and evolutionary selectivity in the small RNA sequences that are released in EVs into the host environment and identifies a novel Argonaute protein as the mediator of this."
Editor
Oxford University Press
Fecha de publicación
2019
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Sugerencia de citación
Franklin Wang-Ngai Chow, Georgios Koutsovoulos, Cesaré Ovando Vázquez, Kyriaki Neophytou, Jose R Bermúdez-Barrientos, Dominik R Laetsch, Elaine Robertson, Sujai Kumar, Julie M Claycomb, Mark Blaxter, Cei Abreu-Goodger, Amy H Buck, Secretion of an Argonaute protein by a parasitic nematode and the evolution of its siRNA guides, Nucleic Acids Research, Volume 47, Issue 7, 23 April 2019, Pages 3594-3606, https://doi.org/10.1093/nar/gkz142
Repositorio Orígen
Repositorio IPICYT
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