Título

Autodisplay of an endo-1,4-β-xylanase from Clostridium cellulovorans in Escherichia coli for xylans degradation

Autor

VICTOR EMMANUEL BALDERAS HERNANDEZ

CARLOS JOEL SALAS MONTANTES

Ana Paulina Barba de la Rosa

Antonio de León Rodríguez

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Identificador alterno

doi: https://doi.org/10.1016/j.enzmictec.2021.109834

Materias

AIDA - (AUTOR) Autodisplay - (AUTOR) Hemicellulose - (AUTOR) Whole cell biocatalysts - (AUTOR) Xylanase - (AUTOR) CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA - (CTI) CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA - (CTI)

Resumen o descripción

"In this work, the scale-up from 0.01 to 10 L process for the co-production of hydrogen (H2) and ethanol (EtOH) by a genetically engineered Escherichia coli that utilizes hemicellulosic hydrolysates from wheat straw pretreated (WSP) as substrate is presented. Co-production of biofuels was performed through the redirection of carbon-flux to ethanol by deleting ldhA (D-lactate dehydrogenase) and frdD (fumarate reductase) genes in an H2-overproducer strain (E. coli WDH). Resulting strain, E. coli WDH-LF (Delta hycA Delta frdD Delta ldhA), increased up to 70% and 167% the H2 and EtOH production from glucose compared with the parenteral strain. Using WSP as substrate, the yields of H2 and EtOH remained constant at all the evaluated scales. In 10 L bioreactors, the production parameters such as maximum production, production rate and yield were 5,603.0 +/- 233.5 mL H2/L, 41.4 +/- 4.0 mL H2/L/h, 342.7 +/- 14.3 mL H2/g TRS, 7.90 +/- 0.28 g EtOH/L and 0.48 +/- 0.01 g EtOH/g TRS, respectively. The results demonstrate the potential of the co-production of H2 and EtOH at different production scales by the engineered E. coli strain using lignocellulosic biomass as feedstock, such as wheat straw hydrolysates."

Editor

Elsevier

Fecha de publicación

2021

Tipo de publicación

Artículo

Versión de la publicación

Versión enviada

Recurso de información

http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2477

Formato

application/pdf

Sugerencia de citación

Victor E. Balderas Hernández, Carlos J. Salas-Montantes, Ana P. Barba-De la Rosa, Antonio De Leon-Rodriguez, Autodisplay of an endo-1,4-β-xylanase from Clostridium cellulovorans in Escherichia coli for xylans degradation, Enzyme and Microbial Technology, Volume 149, 2021, 109834, https://doi.org/10.1016/j.enzmictec.2021.109834.

Repositorio Orígen

Repositorio IPICYT

Descargas

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