Título
Use of in vitro assays to assess the potential antigenotoxic and cytotoxic effects of saffron. (Crocus sativus L.)
Autor
FIKRAT ABDULLAEV JAFAROVA
LETICIA RIVERON NEGRETE
HERIBERTO CABALLERO ORTEGA
JUAN MANUEL HERNANDEZ LOPEZ
ELEAZAR ISRAEL PEREZ LOPEZ
ROGELIO GREGORIO PEREDA MIRANDA
JESUS JAVIER ESPINOSA AGUIRRE
Nivel de Acceso
Acceso Abierto
Materias
MEDICINA Y CIENCIAS DE LA SALUD - (CTI) Antimutagénicos - Toxicidad - ([Toxicology in Vitro 17(5 y 6):731-736]) Supervivencia celular - Efectos de drogas - ([Toxicology in Vitro 17(5 y 6):731-736]) Crocus - Toxicidad - ([Toxicology in Vitro 17(5 y 6):731-736]) Pruebas de mutagenicidad - Métodos - ([Toxicology in Vitro 17(5 y 6):731-736]) Extractos vegetales - Toxicidad - ([Toxicology in Vitro 17(5 y 6):731-736]) Salmonella typhimurium - Genética - ([Toxicology in Vitro 17(5 y 6):731-736]) Células madre - Efectos de drogas - ([Toxicology in Vitro 17(5 y 6):731-736]) Antimutagenic agents - Toxicity - ([Toxicology in Vitro 17(5 y 6):731-736]) Cell Survival - Drug effects - ([Toxicology in Vitro 17(5 y 6):731-736]) Crocus - Toxicity - ([Toxicology in Vitro 17(5 y 6):731-736]) Mutagenicity tests - Methods - ([Toxicology in Vitro 17(5 y 6):731-736]) Plant Extracts - Toxicity - ([Toxicology in Vitro 17(5 y 6):731-736]) Salmonella typhimurium - Genetics - ([Toxicology in Vitro 17(5 y 6):731-736]) Stem Cells - Drug effects - ([Toxicology in Vitro 17(5 y 6):731-736]) Azafrán Crocus sativus - ([Toxicology in Vitro 17(5 y 6):731-736]) Citotoxicidad - ([Toxicology in Vitro 17(5 y 6):731-736]) Mutagenicidad - ([Toxicology in Vitro 17(5 y 6):731-736]) Co-mutagenicidad - ([Toxicology in Vitro 17(5 y 6):731-736]) Saffron Crocus sativus - ([Toxicology in Vitro 17(5 y 6):731-736]) Cytotoxicity - ([Toxicology in Vitro 17(5 y 6):731-736]) Mutagenicity - ([Toxicology in Vitro 17(5 y 6):731-736]) Co-mutagenicity - ([Toxicology in Vitro 17(5 y 6):731-736])
Resumen o descripción
Saffron is harvested from the dried, dark red stigmas of Crocus sativus L. flowers. It is used as a spice for flavoring and coloring food and as a perfume. It is often used for treating several diseases. We assessed the antimutagenic, comutagenic and cytotoxic effects of saffron and its main ingredients using the Ames/Salmonella test system, two well known mutagens (BP, 2AA), the in vitro colony formation assay and four different cultured human normal (CCD-18Lu) and malignant (HeLa, A-204 and HepG2) cells. When only using the TA98 strain in the Ames/Salmonella test system, saffron showed non-mutagenic, as well as non-antimutagenic activity against BP-induced mutagenicity, and demonstrated a dose-dependent co-mutagenic effect on 2-AA-induced mutagenicity. The saffron component responsible for this unusual comutagenic effect was safranal. In the in vitro colony formation test system, saffron displayed a dose-dependent inhibitory effect only against human malignant cells. All isolated carotenoid ingredients of saffron demonstrated cytotoxic activity against in vitro tumor cells. Saffron crocin derivatives possessed a stronger inhibitory effect on tumor cell colony formation. Overall, these results suggest that saffron itself, as well as its carotenoid components might be used as potential cancer chemopreventive agents. © 2003 Elsevier Ltd. All rights reserved.
Editor
Pergamon Press
Fecha de publicación
2003
Tipo de publicación
Artículo
Recurso de información
http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/3112
10.1016/S0887-2333(03)00098-5
Formato
application/pdf
Fuente
Toxicology in Vitro 17(5 y 6):731-736
Idioma
Inglés
Repositorio Orígen
Repositorio del Instituto Nacional de Pediatría
Descargas
292