Título
Effect of dichlorvos on hepatic and pancreatic glucokinase activity and gene expression, and on insulin mRNA levels.
Autor
JOSE GUILLERMO ROMERO NAVARRO
TERESITA GUADALUPE LOPEZ ACEVES
ALBERTO ROJAS OCHOA
MARIA CRISTINA REGINA FERNANDEZ MEJIA
Nivel de Acceso
Acceso Abierto
Materias
MEDICINA Y CIENCIAS DE LA SALUD - (CTI) Diclorvos - Farmacología - Ratas - ([Life Sciences 78(9):1015 - 1020]) Regulación de la expresión génica - Genética - Ratas - ([Life Sciences 78(9):1015 - 1020]) Enzimológica -Efectos de drogas - Ratas - ([Life Sciences 78(9):1015 - 1020]) Glucoquinasa - Metabolismo - Ratas - ([Life Sciences 78(9):1015 - 1020]) Insecticidas - Farmacología - Ratas - ([Life Sciences 78(9):1015 - 1020]) Insulina - Biosíntesis - Ratas - ([Life Sciences 78(9):1015 - 1020]) Dosificación letal mediana - Ratas - ([Life Sciences 78(9):1015 - 1020]) Hígado - Efectos de drogas - Ratas - ([Life Sciences 78(9):1015 - 1020]) Hígado - Enzimología - Ratas - ([Life Sciences 78(9):1015 - 1020]) Páncreas - Efectos de drogas - Ratas - ([Life Sciences 78(9):1015 - 1020]) Páncreas - Enzimología - Ratas - ([Life Sciences 78(9):1015 - 1020]) ARN Mensajero - Biosíntesis - Ratas - ([Life Sciences 78(9):1015 - 1020]) Ratas wistar - ([Life Sciences 78(9):1015 - 1020]) Dichlorvos - Pharmacology - Rats - ([Life Sciences 78(9):1015 - 1020]) Gene expression regulation - Genetics - Rats - ([Life Sciences 78(9):1015 - 1020]) Enzymologic -Drug effects - rats - ([Life Sciences 78(9):1015 - 1020]) Glucokinase - Metabolism - Rats - ([Life Sciences 78(9):1015 - 1020]) Insecticides - Pharmacology - Rats - ([Life Sciences 78(9):1015 - 1020]) Insulin - Biosynthesis - Rats - ([Life Sciences 78(9):1015 - 1020]) Lethal dose 50 - Rats - ([Life Sciences 78(9):1015 - 1020]) Liver - Drug effects - Rats - ([Life Sciences 78(9):1015 - 1020]) Liver - Enzymology - rats - ([Life Sciences 78(9):1015 - 1020]) Pancreas - Drug effects - Rats - ([Life Sciences 78(9):1015 - 1020]) Pancreas - Enzymology - Rats - ([Life Sciences 78(9):1015 - 1020]) RNA, messenger -Biosynthesis - Rats - ([Life Sciences 78(9):1015 - 1020]) Rats, wistar - ([Life Sciences 78(9):1015 - 1020]) Organofosforados - ([Life Sciences 78(9):1015 - 1020]) Plaguicidas - ([Life Sciences 78(9):1015 - 1020]) Diclorvos - ([Life Sciences 78(9):1015 - 1020]) Glucoquinasa - ([Life Sciences 78(9):1015 - 1020]) Insulina - Ratas - ([Life Sciences 78(9):1015 - 1020]) Organophosphate - ([Life Sciences 78(9):1015 - 1020]) Pesticides - ([Life Sciences 78(9):1015 - 1020]) Dichlorvos - ([Life Sciences 78(9):1015 - 1020]) Glucokinase - ([Life Sciences 78(9):1015 - 1020]) Insulin - rats - ([Life Sciences 78(9):1015 - 1020])
Resumen o descripción
Several studies have shown that organophosphate pesticides affect carbohydrate metabolism and produce hyperglycemia. It has been reported that exposure to the organophosphate pesticide dichlorvos affects glucose homeostasis and decreases liver glycogen content. Glucokinase (EC 2.7.1.1) is a tissue-specific enzyme expressed in liver and in pancreatic beta cells that plays a crucial role in glycogen synthesis and glucose homeostasis. In the present study we analyzed the effect of one or three days of dichlorvos administration [20 mg/kg body weight] on the activity and mRNA levels of hepatic and pancreatic glucokinase as well as on insulin mRNA abundance in the rat. We found that the pesticide affects pancreatic and hepatic glucokinase activity and expression differently. In the liver the pesticide decreased the enzyme activity; on the contrary glucokinase mRNA levels were increased. In contrast, pancreatic glucokinase activity as well as mRNA levels were not affected by the treatment. Insulin mRNA levels were not modified by dichlorvos administration. Our results suggest that the decreased activity of hepatic glucokinase may account for the adverse effects of dichlorvos on glucose metabolism. © 2005 Elsevier Inc. All rights reserved.
Editor
Elsevier
Fecha de publicación
2006
Tipo de publicación
Artículo
Recurso de información
http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/3119
10.1016/j.lfs.2005.06.010
Formato
application/pdf
Fuente
Life Sciences 78(9):1015 - 1020
Idioma
Inglés
Repositorio Orígen
Repositorio del Instituto Nacional de Pediatría
Descargas
798