Título
La citotoxicidad inducida por Casiopeína IIgly a las células HeLa agota los niveles de glutatión reducido y es prevenida por el sulfóxido de dimetilo.
Casiopeína IIgly induced cytotoxicity to HeLa cells depletes the levels of reduced glutathione and is prevented by dimethyl sulfoxide.
Autor
FRANCISCO RADAMES ALEMON MEDINA
JOSE LUIS MUÑOZ SANCHEZ
LENA RUIZ AZUARA
MARIA ISABEL GRACIA MORA
Nivel de Acceso
Acceso Abierto
Materias
MEDICINA Y CIENCIAS DE LA SALUD - (CTI) Antineoplásicos - ([Toxicology in Vitro 22(3):710-715]) Antineoplásicos - Toxicidad - ([Toxicology in Vitro 22(3):710-715]) Antioxidantes - Metabolismo - ([Toxicology in Vitro 22(3):710-715]) Muerte celular - Efecto de drogas - ([Toxicology in Vitro 22(3):710-715]) Supervivencia celular - ([Toxicology in Vitro 22(3):710-715]) Citosol - Efectos de drogas - ([Toxicology in Vitro 22(3):710-715]) Citosol - Ultrastructura - ([Toxicology in Vitro 22(3):710-715]) Dimetilsulfóxido - Farmacología - ([Toxicology in Vitro 22(3):710-715]) Colorantes fluorescentes - ([Toxicology in Vitro 22(3):710-715]) Glutation - Metabolismo - ([Toxicology in Vitro 22(3):710-715]) Antineoplastic agents - Antagonists - ([Toxicology in Vitro 22(3):710-715]) & - ([Toxicology in Vitro 22(3):710-715]) inhibitors - ([Toxicology in Vitro 22(3):710-715]) Antineoplastic agents - Toxicity - ([Toxicology in Vitro 22(3):710-715]) Antioxidants - Metabolism - ([Toxicology in Vitro 22(3):710-715]) Cell death - Drug effects - ([Toxicology in Vitro 22(3):710-715]) Cell survival - Drug effects - ([Toxicology in Vitro 22(3):710-715]) Cytosol - Drug effects - ([Toxicology in Vitro 22(3):710-715]) Cytosol - Ultrastructure - ([Toxicology in Vitro 22(3):710-715]) Dimethyl sulfoxide - Pharmacology - ([Toxicology in Vitro 22(3):710-715]) Fluorescent dyes - ([Toxicology in Vitro 22(3):710-715]) Glutathione - Metabolism - ([Toxicology in Vitro 22(3):710-715]) LIgly de la casiopeína - ([Toxicology in Vitro 22(3):710-715]) Daño oxidativo - ([Toxicology in Vitro 22(3):710-715]) Dihydrorhodamine 123 (DHR 123) - ([Toxicology in Vitro 22(3):710-715]) Glutatión reducido (GSH) - ([Toxicology in Vitro 22(3):710-715]) Casiopeína IIgly - ([Toxicology in Vitro 22(3):710-715]) Oxidative damage - ([Toxicology in Vitro 22(3):710-715]) Dihydrorhodamine 123 (DHR 123) - ([Toxicology in Vitro 22(3):710-715]) Reduced glutathione (GSH) - ([Toxicology in Vitro 22(3):710-715])
Resumen o descripción
The newly synthesized copper coordination compound Casiopeína IIgly (Cas IIgly) is a promising alternative drug in the treatment of cancer, since it has shown cytotoxicity and genotoxicity in different tumour models. Given its enhanced effects after ascorbic acid-mediated copper reduction, Cas IIgly's activity is thought to be related to oxidative damage. In the present work, oxidized Cas IIgly failed to induce cytosolic oxidative damage in HeLa cells (only 0.9% of the cell population), and in 2.3% of the treated cells when previously reduced, as evaluated through the oxidation of dihydrorhodamine 123 (DHR 123). However, it showed cytotoxicity, since HeLa cells treated with 10-80 microg/mL Cas IIgly proliferated only at 30% of their normal rate, and at 15% when treated with reduced Cas IIgly. This cytotoxicity is strongly abolished in the presence of the hydroxyl scavenger dimethyl sulfoxide. The decrease, from 3994 to 530 nanograms of reduced glutathione (GSH) per million cells after treatment with 80 microg/mL Casiopeína IIgly, indicates that this drug causes the expenditure of this naturally occurring antioxidant. These results altogether suggest that, albeit Cas IIgly induced cytotoxicity is not related to cytosolic DHR 123 oxidation, it may be related to oxidative damage.
Editor
Pergamon Press
Fecha de publicación
2008
Tipo de publicación
Artículo
Recurso de información
http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/3141
10.1016/j.tiv.2007.11.011
Formato
application/pdf
Fuente
Toxicology in Vitro 22(3):710-715
Idioma
Inglés
Repositorio Orígen
Repositorio del Instituto Nacional de Pediatría
Descargas
247