Título
Cytotoxicity of the β- carboline alkaloids harmine and harmaline in human cell assays in vitro01.
Autor
JUDITH JIMENEZ GUZMAN
LETICIA RIVERON NEGRETE
FIKRAT ABDULLAEV JAFAROVA
JESUS JAVIER ESPINOSA AGUIRRE
ROSARIO RODRIGUEZ ARNAIZ
Nivel de Acceso
Acceso Abierto
Materias
MEDICINA Y CIENCIAS DE LA SALUD - (CTI) Muerte celular - Efectos de drogas - ([Experimental and Toxicologic Pathology 60(4 - 5 ):381-9]) Línea celular tumoral - ([Experimental and Toxicologic Pathology 60(4 - 5 ):381-9]) Proliferación de células - Efectos de drogas - ([Experimental and Toxicologic Pathology 60(4 - 5 ):381-9]) Estimulantes del sistema nervioso central - Toxicidad - ([Experimental and Toxicologic Pathology 60(4 - 5 ):381-9]) Harmalina - Toxicidad - ([Experimental and Toxicologic Pathology 60(4 - 5 ):381-9]) Cell death - Drug effects - ([Experimental and Toxicologic Pathology 60(4 - 5 ):381-9]) Cell line, tumor - ([Experimental and Toxicologic Pathology 60(4 - 5 ):381-9]) Cell proliferation - Drug effects - ([Experimental and Toxicologic Pathology 60(4 - 5 ):381-9]) Central nervous system stimulants - Toxicity - ([Experimental and Toxicologic Pathology 60(4 - 5 ):381-9]) Harmaline - Toxicity - ([Experimental and Toxicologic Pathology 60(4 - 5 ):381-9]) Harmina - Toxicidad - ([Experimental and Toxicologic Pathology 60(4 - 5 ):381-9]) Harmine - Toxicity - ([Experimental and Toxicologic Pathology 60(4 - 5 ):381-9]) Β-carbolina alcaloides - ([Experimental and Toxicologic Pathology 60(4 - 5 ):381-9]) Citocalasina B bloqueado ensayo de micronúcleos (CBMN) - ([Experimental and Toxicologic Pathology 60(4 - 5 ):381-9]) Viabilidad y ensayo de formación de colonia de células - ([Experimental and Toxicologic Pathology 60(4 - 5 ):381-9]) β-Carboline alkaloids - ([Experimental and Toxicologic Pathology 60(4 - 5 ):381-9]) Harmaline - ([Experimental and Toxicologic Pathology 60(4 - 5 ):381-9]) Cytochalasin-B blocked micronucleus (CBMN) assay - ([Experimental and Toxicologic Pathology 60(4 - 5 ):381-9]) Viability and cell colony formation assay - ([Experimental and Toxicologic Pathology 60(4 - 5 ):381-9])
Resumen o descripción
β-Carboline alkaloids are natural products widely distributed in plants and also found in alcoholic beverages, well-cooked foods and tobacco smoke. Various authors have reported genotoxic activities of several carboline in prokaryotic and eukaryotic cells that have been attributed to their abilities to intercalate into DNA. But studies on the genotoxic and on the cytotoxic potencies in human cells in vitro are not found in the literature. In the present study the toxicities of one full aromatic β-carboline alkaloid (harmine) and one dihydro-β-carboline alkaloid (harmaline) were evaluated by means of two in vitro human cell assays: the cytochalasin-B blocked micronucleus (CBMN) assay and the viability/colony formation assay with four different human cultured non-transformed (CCD18Lu) and transformed (HeLa, C33A and SW480) cells. Neither alkaloid was able to induce micronuclei levels above that of control levels in a wide range of doses tested; although, harmine at the highest concentrations assayed induced apoptotic as well as necrotic cells. Harmine produced a good viability of all cell lines assayed (control and tumor) while harmaline significantly reduced the viability of transformed and non-transformed cell lines in a dose-dependent manner. Harmine displayed a dose-dependent inhibitory effect on cell proliferation against all human carcinoma cells, but the SW480 transformed cell line showed a higher sensitivity. These results suggested that harmine was identified as a useful inhibitor of tumor development. © 2008 Elsevier GmbH. All rights reserved.
Editor
Elsevier
Fecha de publicación
2008
Tipo de publicación
Artículo
Recurso de información
http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/3145
10.1016/j.etp.2007.12.003
Formato
application/pdf
Fuente
Experimental and Toxicologic Pathology 60(4 - 5 ):381-9
Idioma
Inglés
Repositorio Orígen
Repositorio del Instituto Nacional de Pediatría
Descargas
839