Título
Effect of temperature on the expression of IFN-1 (α), STAT-1 and Mx-1 genes in Oncorhynchus mykiss (Salmoniformes: Salmonidae) exposed with the virus of the infectious pancreatic necrosis (IPNV)
Autor
Donald Arguedas Cortés
Alex Patricio Romero Zuñiga
Ricardo Enríquez
JOSE SIMON MARTINEZ CASTAÑEDA
CESAR ORTEGA SANTANA
Nivel de Acceso
true
Acceso Abierto
Materias
Biología - ([Revista de Biología Tropical (Costa Rica) Num.2 Vol.63]) IPNV - ([Revista de Biología Tropical (Costa Rica) Num.2 Vol.63]) temperature - ([Revista de Biología Tropical (Costa Rica) Num.2 Vol.63]) rainbow trout - ([Revista de Biología Tropical (Costa Rica) Num.2 Vol.63]) fry - ([Revista de Biología Tropical (Costa Rica) Num.2 Vol.63]) ISGs - ([Revista de Biología Tropical (Costa Rica) Num.2 Vol.63]) real-time PCR - ([Revista de Biología Tropical (Costa Rica) Num.2 Vol.63]) mortality - ([Revista de Biología Tropical (Costa Rica) Num.2 Vol.63]) CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA - (CTI)
Resumen o descripción
The infectious pancreatic necrosis (IPNV) is the causative agent of an acute illness well characterized in salmonids worldwide. Clinical signs and mortality rates are dependent on several factors such as the viral dose, the age of the fish, the water temperature, among others. An experimental study was conducted to measure the effect of temperature on the gene expression profile of IFN-1(α), STAT-1 and Mx-1 in rainbow trout fry, exposed to IPNV. Fry (n=198) were exposed at 8, 12 and 16°C, and samples were taken for 21 days to determine the virus titer and gene expression. In the first 11 days the greatest viral titer was recorded at 8°C compared with the values obtained at 12 and 16°C. At 8°C, there was a significant increase on day 4 of mRNA Mx-1 (t-test, p<0.05), time in which the viral titer began to decrease. Furthermore, as the viral titer increased, STAT-1 and Mx-1 (r=0.91) and (r=0.96) increased, respectively. The animals were able to recover from day 4 from some of the symptoms of IPN. Clinical disease was developed only in fish exposed to 12°C and all died between days 6 and 14, despite the highly significant increase shown in the average expression level of Mx-1, compared with the values recorded at 8°C and 16°C (Tukey, p<0.0001). Additionally, the expression profiles of IFN-1(α) and STAT-1 decreased completely (~0.016) and (~0.020 times) on day 7. The highest expression level of IFN-1(α), occurred at 16°C (Tukey, p<0.0005). Fry exposed at 16°C were normal during the experiment. IFN-1(α) possibly generated a protector effect from day 2 when they showed a significant expression increase compared with the results at 8°C and 12°C (t-student, p<0.0001); however, STAT-1 was not significantly affected by temperature, although the highest average expression value was recorded at 16°C. Our research supports the expression of relevant anti-viral response genes as IFN-1(α), STAT-1 and Mx-1 are physiologically modulated by the water temperature, directly influencing the development of the IPN disease in rainbow trout. Rev. Biol. Trop. 63 (2): 559-569. Epub 2015 June 01.
Editor
Universidad de Costa Rica
Fecha de publicación
2015
Tipo de publicación
Artículo
Recurso de información
Formato
application/application/pdf
Fuente
Revista de Biología Tropical (Costa Rica) Num.2 Vol.63
Idioma
Inglés
Relación
http://www.redalyc.org/revista.oa?id=449
Audiencia
Estudiantes
Investigadores
Repositorio Orígen
REPOSITORIO INSTITUCIONAL DE LA UAEM
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