Título

In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.

Autor

JOSE LUIS PIÑA ESCUTIA

LUIS MIGUEL VAZQUEZ GARCIA

AMAURY MARTIN ARZATE FERNANDEZ

Nivel de Acceso

true

Acceso Abierto

Resumen o descripción

Plants of Tigridia pavonia (L.f.) DC were regenerated from twin-scaling explants cultured on Murashige and Skoog and N6 basal medium. The highest formation of shoots per responding explant was obtained on N6 medium supplemented with 4.5 µM 2,4-dichlorophenoxy acetic acid in combination with 2.2 µM benzylaminopurine. Shoots rooted readily on N6 basal medium supplemented with 1 g l-1 activated charcoal and 2.6 µM naphtalenacetic acid. The rooted shoots achieved 100% survival. Inter Simple Sequence Repeat analysis was carried out to check for possible genetic alterations in plants obtained after two consecutive subcultures. The results revealed that the recovered plants did not exhibit any type of polymorphism.

Editor

Pontificia Universidad Católica de Valparaíso

Fecha de publicación

2010

Tipo de publicación

Artículo

Formato

application/application/pdf

Fuente

Electronic Journal of Biotechnology (Chile) Num.1 Vol.13

Idioma

Inglés

Relación

http://www.redalyc.org/revista.oa?id=1733

Audiencia

Estudiantes

Investigadores

Repositorio Orígen

REPOSITORIO INSTITUCIONAL DE LA UAEM

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