Título
Variety discrimination of Tigridia pavonia (L.f.) DC. assesed by different length RAPD primers
Autor
JOSE LUIS PIÑA ESCUTIA
LUIS MIGUEL VAZQUEZ GARCIA
AMAURY MARTIN ARZATE FERNANDEZ
Nivel de Acceso
true
Acceso Abierto
Materias
Biología - ([Electronic Journal of Biotechnology (Chile) Num.4 Vol.13]) Genetic resources - ([Electronic Journal of Biotechnology (Chile) Num.4 Vol.13]) RAPD - ([Electronic Journal of Biotechnology (Chile) Num.4 Vol.13]) Tigridia pavonia - ([Electronic Journal of Biotechnology (Chile) Num.4 Vol.13]) variety discrimination - ([Electronic Journal of Biotechnology (Chile) Num.4 Vol.13]) BIOLOGÍA Y QUÍMICA - (CTI)
Resumen o descripción
Tigridia pavonia (L.f.) DC. is one of the important phytogenetic resources of México. This species is used as ornamental, food and medicinal purposes. Despite its ornamental and economic potential, there is little information about the genetic variability. In this study, randomly amplified polymorphic DNA (RAPD) primers of 10, 15 and 20 bases were used to assess the level of genetic variation among nine botanical varieties of Tigridia pavoniacollected in three localities within State of México. The total number fragments, polymorphic fragments, percentage of polymorphism and resolving power were greater for 15 base (55, 52, 94.5 and 5, respectively) and 20 base (47, 45, 95.7 and 3.8, respectively), in comparison with those obtained from 10 base primers (44, 41, 93.1 and 3.6, respectively).Resultsshowed the major effectiveness of 15 and 20 bases RAPD primers in the genetic differentiation of varieties as compared to 10 bases RAPD primers. The dendrograms based on un-weighted pair group method arithmetic average (UPGMA) analysis of the 10, 15, 20 and the pooled (10, 15 and 20) bases RAPD data were consistent in the clustering varieties, grouping them in two main clusters.
Editor
Pontificia Universidad Católica de Valparaíso
Fecha de publicación
2010
Tipo de publicación
Artículo
Recurso de información
Formato
application/application/pdf
Fuente
Electronic Journal of Biotechnology (Chile) Num.4 Vol.13
Idioma
Inglés
Relación
http://www.redalyc.org/revista.oa?id=1733
Audiencia
Estudiantes
Investigadores
Repositorio Orígen
REPOSITORIO INSTITUCIONAL DE LA UAEM
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