Título

Anti-staphylococcal properties of Eichhornia crassipes, Pistacia vera, and Ziziphus amole leaf extracts: Isolates from cattle and rabbits

Autor

ANDREA GUTIERREZ MORALES

VALENTE VELAZQUEZ ORDOÑEZ

AMEER KHUSRO

Abdelfattah Zeidan Mohamed Salem

MARIA ELENA ESTRADA ZUÑIGA

MOHAMED ZEIDAN MOHAMED SALEM

BENJAMIN VALLADARES CARRANZA

CRISTINA BURROLA AGUILAR

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Materias

E. crassipes - ([0882-4010]) Extract - ([0882-4010]) P. vera - ([0882-4010]) Staphylococci - ([0882-4010]) Z. amole - ([0882-4010]) CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA - (CTI)

Resumen o descripción

Eichhornia crassipes (Water hyacinth), belonging to the family Pontideriaceae is one of the most productive aquatic perennial herbs on earth, and it has been known for its unique medicinal importance. The phytoconstituents of this plant have vast biological properties including antiviral, antifungal, antitumor, and antibacterial activities [7]. Additionally, its secondary metabolites have been considered to be involved in the chemical defense of plants against plant pathogens

The desideratum aim of the present context was to assess the biopotency of methanolic extracts of Eichhornia crassipes (E. crassipes), Pistacia vera (P. vera), and Ziziphus amole (Z. amole) leaves against various staphylococcal strains, and to quantify the phenolics as well as saponin content in them. The antibacterial activity of various concentrations (62.5–1000 μg/mL) of plant extracts was tested against control clinical strains (Staphylococcus aureus ATCC 25923, S. aureus ATCC 29213, and S. aureus ATCC 43300), methicillin-resistant S. aureus (MRSA1 and MRSA2), oxacillin sensitive S. aureus (SOSA1 and SOSA2), and coagulase-negative Staphylococcus epidermidis (CoNS1, CoNS2, and CoNS3) using disc diffusion assay. Leaf extracts of the three plants exhibited pronounced growth inhibitory characteristics against staphylococci in a dose dependent manner. E. crassipes extract depicted the highest relative percentage inhibition values against control clinical strains (68.6 ± 0.5%), while P. vera (68.6 ± 0.3%) and Z. amole (74.79 ± 0.3%) extracts showed pronounced relative inhibition values against staphylococcal strains isolated from cattle. Total phenols and saponin content of leaf extracts were investigated by standard in vitro methods. The methanolic extracts of these plants were found to comprise substantial content of phenolics and saponin at varying levels. The highest value of phenolics was estimated in P. vera extract (60.0 ± 1.3 mg gallic acid/g extract), followed by Z. amole (33.6 ± 1.4 mg gallic acid/g extract), and E. crassipes (23.0 ± 1.3 mg gallic acid/g extract). Saponin content for P. vera, Z. amole, and E. crassipes extracts were estimated as 41.0 ± 1.3, 35.8 ± 1.3, and 25.0 ± 1.2 mg diosgenin/g extract, respectively. The outcome of this study suggested the exploitation of methanolic extract of P. vera, Z. amole, and E. crassipes leaves for their possible application in ethnomedicine, particularly as drugs preparation against staphylococcal infections. In conclusion, the study indicates the biopotency of these plants against pathogenic MRSA present in cattle, and SOSA as well as CoNS bacteria present in rabbits, which could be a serious issue for livestock.

Editor

ELSEVIER

Fecha de publicación

1 de febrero de 2017

Tipo de publicación

Artículo

Recurso de información

http://hdl.handle.net/20.500.11799/67634

Fuente

0882-4010

Idioma

Inglés

Relación

113;

Audiencia

Estudiantes

Investigadores

Repositorio Orígen

REPOSITORIO INSTITUCIONAL DE LA UAEM

Descargas

353