Título
Respiratory syncytial virus glycoproteins uptake occurs through clathrin-mediated endocytosis in a human epithelial cell line.
Autor
Abel Gutierrez Ortega
Carla Vanessa Sanchez Hernandez
Beatriz Gomez Garcia
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: 10.1186/1743-422X-5-127
Materias
Virología - ([Virology Journal (1743-422X ) vol.5(1), 1-4 (2008)]) Virus sincitial respiratorio - ([Virology Journal (1743-422X ) vol.5(1), 1-4 (2008)]) Infección - ([Virology Journal (1743-422X ) vol.5(1), 1-4 (2008)]) Internalización de antígeno - ([Virology Journal (1743-422X ) vol.5(1), 1-4 (2008)]) BIOLOGÍA Y QUÍMICA - (CTI)
Resumen o descripción
Cell-surface viral proteins most frequently enter the cell through clathrin or caveolae endocytosis. Respiratory syncytial virus antigen internalization by immune cells is via caveolin, however, uptake of paramyxovirus cell membrane proteins by non-immune cells is done through clathrin-coated pits. In this work, the uptake of respiratory syncytial virus cell surface glycoproteins by non-immune human epithelial cells was investigated through indirect immunofluorescence with polyclonal anti-RSV antibody and confocal lasser-scanner microscopy. Clathrin and caveolae internalization pathways were monitored through specific inhibitors monodansylcadaverine (MDC) and methyl-beta-cyclodextrin (MBCD), respectively. Internalization of RSV antigens was inhibited by MDC but not by MBCD, implying that clathrin-mediated endocytosis is the major uptake route of RSV antigens by an epithelial human cell line.
Editor
BioMed Central
Fecha de publicación
2008
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
Virology Journal (1743-422X ) vol.5(1), 1-4 (2008)
Idioma
Inglés
Relación
https://virologyj.biomedcentral.com/articles?query=
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Cobertura
GB
Repositorio Orígen
Repositorio Institucional de la Facultad de Medicina,UNAM.Departamento de Microbiologia y Parasitologia
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