Author: ABEL GUTIERREZ ORTEGA

Anticuerpos biespecíficos: la nueva generación de anticuerpos terapéuticos

Abel Gutiérrez Ortega (2019)

El progreso que se ha dado en el campo de la medicina ha tenido, indudablemente, un impacto muy positivo en la calidad y expectativa de vida de los seres humanos, con lo que hoy en día se cuenta con moléculas para el tratamiento de diversas enfermedades crónico-degenerativas, como la diabetes y el cáncer. Dentro de estas moléculas, se encuentran los anticuerpos monoclonales.

Book part

Anticuerpos MEDICINA Y CIENCIAS DE LA SALUD CIENCIAS MÉDICAS OTRAS ESPECIALIDADES MÉDICAS OTRAS ESPECIALIDADES MÉDICAS

Péptido recombinante de la porina de 42-kDa, uso como antígeno vacunal y en la detección de anticuerpos contra Histophilus somni

RODOLFO HERNANDEZ GUTIERREZ Abel Gutiérrez Ortega (2020)

En la presente invención se solicita un método para producir una respuesta inmune contra Histophilus somni en un animal mediante la administración de un péptido recombinante que corresponde a una porina de 42 kDa con una secuencia de los aminoácidos 129 al 339. También se reclama una formulación de una vacuna contra Histophilus somni que comprende un vehículo farmacéuticamente aceptable y un péptido recombinante que corresponde a una porina de 42 kDa con una secuencia de aminoaácidos del 129 al 339.

Patent

BIOLOGÍA Y QUÍMICA BIOLOGÍA Y QUÍMICA

Identification of immunodominant proteins from Mannheimia haemolytica and Histophilus somni by an immunoproteomic approach

RODOLFO HERNANDEZ GUTIERREZ ANGEL HILARIO ALVAREZ HERRERA ABEL GUTIERREZ ORTEGA (2015)

"Mannheimia haemolytica and Histophilus somni are frequently isolated from diseased cattle with bovine respiratory disease (BRD).

They compromise animal lung function and the immune responses generated are not sufficient to limit infection. Identification of specific immunogenic antigens for vaccine development represents a great challenge. Immunogenic proteins were identified by immunoproteomic approach with sera from cattle immunized with a commercial cellular vaccine of M. haemolytica and H. somni.

Proteins of M. haemolytica were identified as solute ABC transporter, iron-binding protein, and hypothetical protein of capsular

biosynthesis. Histophilus somni proteins correspond to porin, amino acid ABC transporter, hypothetical outer membrane protein,

cysteine synthase, and outer membrane protein P6. Although these antigens share strong similarities with other proteins from animal pathogens, the ABC system proteins have been associated with virulence and these proteins could be considered as potential vaccine candidates for BRD."

Article

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Respiratory syncytial virus glycoproteins uptake occurs through clathrin-mediated endocytosis in a human epithelial cell line.

Abel Gutierrez Ortega Carla Vanessa Sanchez Hernandez Beatriz Gomez Garcia (2008)

Cell-surface viral proteins most frequently enter the cell through clathrin or caveolae endocytosis. Respiratory syncytial virus antigen internalization by immune cells is via caveolin, however, uptake of paramyxovirus cell membrane proteins by non-immune cells is done through clathrin-coated pits. In this work, the uptake of respiratory syncytial virus cell surface glycoproteins by non-immune human epithelial cells was investigated through indirect immunofluorescence with polyclonal anti-RSV antibody and confocal lasser-scanner microscopy. Clathrin and caveolae internalization pathways were monitored through specific inhibitors monodansylcadaverine (MDC) and methyl-beta-cyclodextrin (MBCD), respectively. Internalization of RSV antigens was inhibited by MDC but not by MBCD, implying that clathrin-mediated endocytosis is the major uptake route of RSV antigens by an epithelial human cell line.

Article

Virología Virus sincitial respiratorio Infección Internalización de antígeno BIOLOGÍA Y QUÍMICA

Temas de interés en Salud Humana que se están abordando en la Unidad de Biotecnología Médica y Farmacéutica del Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C.

FLOR YOHANA FLORES HERNANDEZ MARIO ALBERTO FLORES VALDEZ Abel Gutiérrez Ortega (2019)

La Unidad de Biotecnología Médica y Farmacéutica del Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ) está conformada por 22 integrantes con un alto grado de especialización que realizan proyectos de investigación y desarrollo, así como la prestación de servicios, con una fuerte orientación a atender las necesidades en el país que están relacionadas con la salud humana y animal, empleando tecnología avanzada para ello. Las temáticas que atiende la Unidad son muy diversas, pero todas transitan en torno a cuatro ejes de investigación, desde cómo tratar o corregir una enfermedad, cómo detectarla de

manera oportuna y sencilla, hasta cómo prevenirla:

1. Desarrollo y evaluación de vacunas y compuestos inmunomoduladores.

2. Desarrollo y validación de pruebas de diagnóstico molecular.

3. Desarrollo y evaluación de productos con potencial terapéutico.

4. Ingeniería biomédica de medicamentos biotecnológicos e ingeniería de tejidos.

Book

Salud MEDICINA Y CIENCIAS DE LA SALUD CIENCIAS MÉDICAS CIENCIAS MÉDICAS

Immune response to a potyvirus with exposed amino groups available for chemical conjugation

CARLOS ALBERTO MANUEL CABRERA ANA LAURA MARQUEZ AGUIRRE RODOLFO HERNANDEZ GUTIERREZ Pablo César Ortiz Lazareno Gabriela Chávez Calvillo MAURICIO CARRILLO TRIPP LAURA SILVA ROSALES Abel Gutiérrez Ortega (2012)

"Background:

The amino terminus of the tobacco etch virus (TEV) capsid protein is located on the external surface of infectious TEV particles, as proposed by previous studies and an in silico model. The epsilon amino groups on the exposed lysine residues are available for chemical conjugation to any given protein, and can thus act as antigen carriers. The availability of amino groups on the surfaces of TEV particles was determined and the immune response to TEV evaluated.

Results:

Using a biotin-tagged molecule that reacts specifically with amino groups, we found that the TEV capsid protein has amino groups on its surface available for coupling to other molecules via crosslinkers. Intraperitoneal TEV was administered to female BALB/c mice, and both their humoral and cellular responses measured. Different IgG isotypes, particularly IgG2a, directed against TEV were induced. In a cell proliferation assay, only spleen cells from vaccinated mice that were stimulated in vitro with TEV showed significant proliferation of CD3+/CD4+ and CD3+/CD8+ subpopulations and secreted significant amounts of interferon g.

Conclusions:

TEV has surface amino groups that are available for chemical coupling. TEV induces both humoral and cellular responses when administered alone intraperitoneally to mice. Therefore, TEV should be evaluated as a vaccine adjuvant when chemically coupled to antigens of choice.

Keywords: Tobacco etch virus, capsid protein, amino groups, chemical conjugation, immune response."

Article

BIOLOGÍA Y QUÍMICA BIOLOGÍA Y QUÍMICA

Self-assembly of hexahistidine-tagged tobacco etch virus capsid protein into microfilaments that induce IgG2-specific response against a soluble porcine reproductive and respiratory syndrome virus chimeric protein

CARLOS ALBERTO MANUEL CABRERA ALBA ADRIANA VALLEJO CARDONA EDUARDO PADILLA CAMBEROS RODOLFO HERNANDEZ GUTIERREZ SARA ELISA HERRERA RODRIGUEZ ABEL GUTIERREZ ORTEGA (2016)

"Abstract

Background: Assembly of recombinant capsid proteins into virus-like particles (VLPs) still represents an interesting

challenge in virus-based nanotechnologies. The structure of VLPs has gained importance for the development and

design of new adjuvants and antigen carriers. The potential of Tobacco etch virus capsid protein (TEV CP) as adjuvant

has not been evaluated to date.

Findings: Two constructs for TEV CP expression in Escherichia coli were generated: a wild-type version (TEV-CP) and a

C-terminal hexahistidine (His)-tagged version (His-TEV-CP). Although both versions were expressed in the soluble

fraction of E. coli lysates, only His-TEV-CP self-assembled into micrometric flexuous filamentous VLPs. In addition,

the His-tag enabled high yields and facilitated purification of TEV VLPs. These TEV VLPs elicited broader IgG2-

specific antibody response against a novel porcine reproductive and respiratory syndrome virus (PRRSV) protein

when compared to the potent IgG1 response induced by the protein alone.

Conclusions: His-TEV CP was purified by immobilized metal affinity chromatography and assembled into VLPs,

some of them reaching 2-μm length. TEV VLPs administered along with PRRSV chimeric protein changed the

IgG2/IgG1 ratio against the chimeric protein, suggesting that TEV CP can modulate the immune response against

a soluble antigen".

Article

virus del grabado del tabaco, proteína de la cápside, partículas pseudovíricas, etiqueta de hexahistidinas, adyuvante, proteína quimérica BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR

Immune response to a potyvirus with exposed amino groups available for chemical conjugation

CARLOS ALBERTO MANUEL CABRERA ANA LAURA MARQUEZ AGUIRRE RODOLFO HERNANDEZ GUTIERREZ PABLO CESAR ORTIZ LAZARENO GABRIELA CHAVEZ CALVILLO MAURICIO CARRILLO TRIPP LAURA SILVA ROSALES ABEL GUTIERREZ ORTEGA (2017)

"Background: The amino terminus of the tobacco etch virus (TEV) capsid protein is located on the external surface of infectious TEV particles, as proposed by previous studies and an in silico model. The epsilon amino groups on the exposed lysine residues are available for chemical conjugation to any given protein, and can thus act as antigen carriers. The availability of amino groups on the surfaces of TEV particles was determined and the immune response to TEV evaluated.

Results: Using a biotin-tagged molecule that reacts specifically with amino groups, we found that the TEV capsid protein has amino groups on its surface available for coupling to other molecules via crosslinkers. Intraperitoneal TEV was administered to female BALB/c mice, and both their humoral and cellular responses measured. Different IgG isotypes, particularly IgG2a, directed against TEV were induced. In a cell proliferation assay, only spleen cells from vaccinated mice that were stimulated in vitro with TEV showed significant proliferation of CD3+/CD4+ and CD3+/CD8+ subpopulations and secreted significant amounts of interferon g.

Conclusions: TEV has surface amino groups that are available for chemical coupling. TEV induces both humoral and cellular responses when administered alone intraperitoneally to mice. Therefore, TEV should be evaluated as a vaccine adjuvant when chemically coupled to antigens of choice".

Article

BIOLOGÍA Y QUÍMICA

Mycobacterium tuberculosis TLR-2 agonists LprA, LM and Man-LAM induce notch1 and socs3 transcription

MARIO ALBERTO FLORES VALDEZ VANIA ELVIRA BONIFAZ PENA ERIKA NAHOMY MARINO MARMOLEJO CITLALLI TORNEZ BENITEZ Mabel Guzmán Rodríguez Abel Gutiérrez Ortega Letícia Santos (2011)

"Mycobacterium tuberculosis employs a number of strategies to subvert host signaling events, leading to its persistence within macrophages. Upon infection, Mycobacterium bovis BCG induce the expression of suppressor of cytokine signaling 3 (socs3), in a Toll-like receptor 2 (TLR2)-Notch1- dependent manner. Purified phosphatidyl inositol di-mannosides (a TLR2 agonist) act as an inducer for the Notch1-socs3 pathway. This prompted us to analyze other TLR2 agonists seeking for additional molecules that may affect this pathway. We found that lipoprotein LprA, as well as glycolipids lipomannan (LM), and mannose-capped lipoarabinomannan (ManLAM) treatment of murine macrophages resulted in stimulation of notch1 and socs3 transcription".

Article

BIOLOGÍA Y QUÍMICA BIOLOGÍA Y QUÍMICA

Detection of dengue, west Nile virus, rickettsiosis and leptospirosis by a new real-time PCR strategy

DARWIN EDUARDO ELIZONDO QUIROGA ALBERTINA CARDENAS VARGAS ERIKA NAHOMY MARINO MARMOLEJO ABEL GUTIERREZ ORTEGA ESTEBAN GONZALEZ DIAZ MARIA DEL RAYO MORFIN OTERO EDUARDO RODRIGUEZ NORIEGA DANIEL GARCIA RUIZ MARCO ALONSO MARTINEZ GUZMAN HECTOR RAUL PEREZ GOMEZ (2016)

"Dengue virus (DENV) infection causes sudden fever along with several nonspecific signs and symptoms and in severe cases, death. DENV is transmitted to people by Aedes aegypti and Ae. albopictus mosquitoes, whose populations increase during rainy season. West Nile Virus (WNV), Rickettsia spp. and Leptospira spp. are fever-causing pathogens that share many of the initial symptoms of DENV infection and also thrive in the rainy season. Outbreaks in some regions may be due to any of these pathogens that can co-circulate. Plus, they are clinically indistinguishable until severe symptoms appear, even though these diseases should be treated differently. An effective differential diagnosis would help clinicians and vector control departments to make right decisions for control and treatment of these diseases. Therefore, we developed four different SYBR green®-based reverse transcription quantitative PCR (RT-qPCR) assays for simultaneous detection of DENV, WNV, Rickettsia spp. and Leptospira spp. The assay has been optimized to yield results in less than 1 h; and in order to reduce contamination risk, all reagents were premixed and lyophilized on 96 well plates and thus only requires the addition of water and total nucleic acids from the sample. Sensitivities of the assays were less than 100 copies of nucleic acid targeted for these four pathogens. Assays did not show cross reactivity with any of the four pathogens nor to human nucleic acids. We are presenting a sensitive and selective kit that detects four relevant pathogens from tropical regions, that is quick, cost-effective and easy to use."

Article

BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA VIROLOGÍA ARBOVIRUS