Author: ALBERTINA CARDENAS VARGAS

Detection of dengue, west Nile virus, rickettsiosis and leptospirosis by a new real-time PCR strategy

DARWIN EDUARDO ELIZONDO QUIROGA ALBERTINA CARDENAS VARGAS ERIKA NAHOMY MARINO MARMOLEJO ABEL GUTIERREZ ORTEGA ESTEBAN GONZALEZ DIAZ MARIA DEL RAYO MORFIN OTERO EDUARDO RODRIGUEZ NORIEGA DANIEL GARCIA RUIZ MARCO ALONSO MARTINEZ GUZMAN HECTOR RAUL PEREZ GOMEZ (2016)

"Dengue virus (DENV) infection causes sudden fever along with several nonspecific signs and symptoms and in severe cases, death. DENV is transmitted to people by Aedes aegypti and Ae. albopictus mosquitoes, whose populations increase during rainy season. West Nile Virus (WNV), Rickettsia spp. and Leptospira spp. are fever-causing pathogens that share many of the initial symptoms of DENV infection and also thrive in the rainy season. Outbreaks in some regions may be due to any of these pathogens that can co-circulate. Plus, they are clinically indistinguishable until severe symptoms appear, even though these diseases should be treated differently. An effective differential diagnosis would help clinicians and vector control departments to make right decisions for control and treatment of these diseases. Therefore, we developed four different SYBR green®-based reverse transcription quantitative PCR (RT-qPCR) assays for simultaneous detection of DENV, WNV, Rickettsia spp. and Leptospira spp. The assay has been optimized to yield results in less than 1 h; and in order to reduce contamination risk, all reagents were premixed and lyophilized on 96 well plates and thus only requires the addition of water and total nucleic acids from the sample. Sensitivities of the assays were less than 100 copies of nucleic acid targeted for these four pathogens. Assays did not show cross reactivity with any of the four pathogens nor to human nucleic acids. We are presenting a sensitive and selective kit that detects four relevant pathogens from tropical regions, that is quick, cost-effective and easy to use."

Article

BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA VIROLOGÍA ARBOVIRUS

AVANCES EN LA SEGURIDAD Y ACTIVIDAD BIOLÓGICA DE SUSTANCIAS BIOACTIVAS Y PROBIÓTICOS

HUGO ESPINOSA ANDREWS Zaira Yunuen García Carvajal ERISTEO GARCIA MARQUEZ ORFIL GONZALEZ REYNOSO ROBERTO OLIVARES HERNANDEZ María del Refugio Ramos_Jerz CLAUDIA YURITZI FIGUEROA HERNANDEZ Rodrigo Alonso Villegas ERIKA NAHOMY MARINO MARMOLEJO MARISELA GONZALEZ AVILA SARA ELISA HERRERA RODRIGUEZ FLOR YOHANA FLORES HERNANDEZ Eduardo Padilla Camberos Jorge Bravo Madrigal MOISES MARTINEZ VELAZQUEZ RODOLFO HERNANDEZ GUTIERREZ EVANGELINA ESMERALDA QUIÑONES AGUILAR Gabriel Rincón Enríquez JUDITH ESMERALDA URIAS SILVAS LUIS ALFONSO MOJICA CONTRERAS SOCORRO JOSEFINA VILLANUEVA RODRIGUEZ José Daniel Padilla de la Rosa BRENDA SAN PABLO OSORIO JESUS RAFAEL TRINIDAD CRUZ GLADYS PAEZ HERNANDEZ ROSA JARUMY LOPEZ RIVERA ALBERTINA CARDENAS VARGAS María Gretel Michel Barba KARINA ESMERALDA ENRIQUEZ RAMIREZ Alma Rosa Oaxaca Camacho (2017)

Los compuestos bioactivos son componentes esenciales y no esenciales (por ejemplo, vitaminas o polifenoles, prebióticos, probióticos, etc.) que se producen en la naturaleza, son parte de la cadena alimentaria y pueden demostrar que tienen un efecto sobre la salud humana. La dieta no sólo proporciona nutrientes adecuados para satisfacer los requisitos metabólicos, sino que también puede contribuir a la mejora de la salud humana. En consecuencia, los compuestos bioactivos que se cree que benefician a la salud humana necesitan ser identificados y desarrollados para el mercado de alimentos para complementar una dieta equilibrada. La evaluación de la eficacia y seguridad de estos compuestos bioactivos naturales representa un desafío para los científicos.

Book

Probióticos; Sustancias bioactivas INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS TECNOLOGÍA DE LOS ALIMENTOS PROPIEDADES DE LOS ALIMENTOS PROPIEDADES DE LOS ALIMENTOS