Author: ALBERTO BARBABOSA PLIEGO

Outbreak of Zika virus pathogenesis and quest of its vaccine development: Where do we stand now?

AMEER KHUSRO Chirom Aarti ALBERTO BARBABOSA PLIEGO PEDRO SANCHEZ APARICIO (2018)

Zika virus (ZIKV) infection is a life-threatening tropical infection, mainly caused by mosquito bite. After a very long period of quietness, ZIKV infections have become a problematic issue again. Previously, the virus was limited to Africa and Asia only but later it emerged in Brazil, South America, and other parts of the world in 2015. In 2016, there are emerging new cases of sexually transmitted ZIKV infection as well. At present, there is no proper treatment and available pronounced vaccines for the treatment of ZIKV infection. The prime focal point of this review is not only to provide imperative epidemiological information on ZIKV infection in brief but also the current situation of vaccines testing on animal model as well as in clinical trial phases. Currently there is no human vaccine for this pestiferous viral infection. Therefore, prevention, proper management, and up-to-date recommendation are crucial to mitigate the possible risk of vector and non-vector transmission of ZIKV.

Article

Tropical infection Vaccine Viral infection Zika virus MEDICINA Y CIENCIAS DE LA SALUD

Influence of nisin and lauryl arginine ester against some foodborne pathogens in recombined feta and processed spread cheese

TAREQ ALNEMR SAMEH MOHAMED ALBERTO BARBABOSA PLIEGO ABDELFATTAH ZEIDAN MOHAMED SALEM (2015)

The aim of this study was to evaluate the impact of nisin with lauryl arginine ester (LAE) or their combination by 1: 3 (w/w) on cheese preservation. The study was carried out against the most common foodborne pathogens in recombined feta cheese (RFC) and processed cheese spread (PCS). Combination of nisin with LAE has higher synergistic preservative effect on different widespread foodborne pathogens such as Bacillus subtilis, Clostridium sporogenes and Escherichia coli compared with individual one. The most sensitive strain was E. coli with an effective minimal inhibition concentration (MIC) of 400 ppm, whereas the former spore-forming bacterial strains were totally inhibited using 700 ppm from the combination, respectively. Synergistic combination blend was added by its recommended MIC (700 ppm) in the manufacture of both RFC and PCS. The results indicated that it is efficient enough to inhibit the growth of the most common foodborne pathogens in cheese after their storage for 7 days (RFC) and 30 days (PCS).

Article

foodborne pathogens cheese lauryl arginine ester CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Influence of Aguamiel (Agave atrovirens) as a Natural Feed Additive on Cecal Fermentation Kinetics of Some Forage Species in Horse Feeding

ABDELFATTAH ZEIDAN MOHAMED SALEM Nestor Torres Olurotimi Olafadehan ALBERTO BARBABOSA PLIEGO ROSALIA LUGO COYOTE (2016)

The ability of the horse to efficiently utilize fiber and roughages due to the presence of fermentative microorganisms in their hindgut and the use of fibrous feeds as the main component of the mature horse diet have been documented [1,2]. Forages are important primary natural component of horse diet needed for normal function of their digestive system and to suppress certain metabolic disorders like hindgut acidosis, laminitis, and colic occasioned by feeding high-starch diets [3]

This study aimed to evaluate the effect of different dose levels of aguamiel (Agave atrovirens) on in vitro cecal gas, methane (CH4), and carbon dioxide (CO2) productions of five forage species (Avena sativa [hay]), Moringa oleifera, Caesalpinia coriacea, Salix babylonica, and Eichhornia crassipes using inocula from the horse. The forage samples were incubated with three doses of aguamiel: 0, 34, and 68 mg of aguamiel/g dry matter (DM) of substrate. Cecal inocula were collected from four adult female Criolla horses (3–4 years of age and weighing 300 15.0 kg) grazed on native grasses for about 8 hours without supplementation. Forage type affected (P <.001) cecal asymptotic, rate and lag time of gas, CH4 and CO2 productions (mL/g DM), pH and DM degradability. Aguamiel dose had linear and quadratic effects (P < .05) on the asymptotic and rate of CH4 productions and rate and lag time of CO2 productions (mL/g DM). Forage type aguamiel dose interactions were significant (P < .05) for asymptotic, rate and lag time of gas, and CH4 and CO2 productions (mL/g DM). Forage species effects were pronounced (P < .05) on CH4 and CO2 productions (mL/g incubated and degraded DM) and proportional CH4 production at all hours of incubation, except for CO2 production (mL/g incubated DM). Aguamiel dose affected (P <.05) CO2 production (mL/g incubated DM) and proportional CO2 production at the incubated hours. Forage type aguamiel dose interactions were observed (P < .05) for CO2 production (mL/g incubated DM) and proportional CO2 production at the incubated hours but had no impact on CH4 production. It is concluded that addition of aguamiel to five forage species affected fermentation kinetics of gas production resulting in different in vitro cecal gas, CH4 and CO2 productions from these substrates

Article

aguamiel cecal fermentation forage gas production methane carbon dioxide CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Influence of Feeding Horses a High Fiber Diet With or Without Live Yeast Cultures Supplementation on Feed Intake, Nutrient Digestion, Blood Chemistry, Fecal Coliform Count, and In Vitro Fecal Fermentation

Abdelfattah Zeidan Mohamed Salem MONA MOHAMED MOHAMED YASSEEN ELGHANDOUR Ahmed E. Kholif ALBERTO BARBABOSA PLIEGO LUIS MIGUEL CAMACHO DIAZ NICHOLAS E. ODONGO (2015)

Sixteen Quarter Horse mares (body weight: 450–500 kg) were used in a complete randomized design to determine the effects of feeding horses a high fiber diet with or without yeast cultures addition on nutrient intake and digestion, blood chemistry, fecal coliform count, and in vitro fecal fermentation. The treatments were (1) a basal diet without yeast cultures addition (control treatment), (2) control diet plus Procreatin 7 at 15 g/mare/d (P7 treatment), (3) control diet plus Biocell F53 at 11 g/mare/d (F53 treatment), or (4) control diet plus Biosaf SC47 at 15 g/mare/d (SC47 treatment). The basal concentrate diet consisted of a mixture of 50% commercial concentrates and 50% wheat bran fed at 4 kg/mare and offered twice daily at 04:00 and 16:00 hours,while oat strawwas offered ad libitumat 05:00 and 17:00 hours. The mares fed the F53 had higher (P < .05) oat straw and total nutrient intakes compared to the control diet. Addition of Biocell F53 and Biosaf SC47 yeast cultures increased (P <.05) all nutrients’ digestibilities. Feeding the yeast cultures resulted in higher crude protein (P¼.029), neutral detergent fiber (P¼.042), and acid detergent fiber (P¼.035) digestibilities compared to the control diet. The SC47 treatment had lower blood total protein (P ¼.014) than the control treatment. Higher (P <.05) asymptotic in vitro fecal gas production was obtained with F53 treatment compared to SC47 treatment without differences between F53, P7, and control treatments. Increased methane productionwas obtained (P < .05) with F53 and SC47 treatments compared to the control treatment. It can be concluded that daily addition of Biocell F53 yeast culture at 11 g/mare/d resulted in higher feed intake and nutrients digestibility without affecting the mare’s health.

Article

Coliform Feed utilization Horse Yeast culture BIOLOGÍA Y QUÍMICA

Influence of Aguamiel (Agave atrovirens) as a Natural Feed Additive on Cecal Fermentation Kinetics of Some Forage Species in Horse Feeding

ABDELFATTAH ZEIDAN MOHAMED SALEM Nestor Torres Olurotimi Olafadehan MONA MOHAMED MOHAMED YASSEEN ELGHANDOUR ALBERTO BARBABOSA PLIEGO ROSALIA LUGO COYOTE (2017)

This study aimed to evaluate the effect of different dose levels of aguamiel (Agave atrovirens) on in vitro cecal gas, methane (CH4), and carbon dioxide (CO2) productions of five forage species (Avena sativa [hay]), Moringa oleifera, Caesalpinia coriacea, Salix babylonica, and Eichhornia crassipes using inocula from the horse. The forage samples were incubated with three doses of aguamiel: 0, 34, and 68 mg of aguamiel/g dry matter (DM) of substrate. Cecal inocula were collected from four adult female Criolla horses (3–4 years of age and weighing 300 15.0 kg) grazed on native grasses for about 8 hours without supplementation. Forage type affected (P <.001) cecal asymptotic, rate and lag time of gas, CH4 and CO2 productions (mL/g DM), pH and DM degradability. Aguamiel dose had linear and quadratic effects (P < .05) on the asymptotic and rate of CH4 productions and rate and lag time of CO2 productions (mL/g DM). Forage type aguamiel dose interactions were significant (P < .05) for asymptotic, rate and lag time of gas, and CH4 and CO2 productions (mL/g DM). Forage species effects were pronounced (P < .05) on CH4 and CO2 productions (mL/g incubated and degraded DM) and proportional CH4 production at all hours of incubation, except for CO2 production (mL/g incubated DM). Aguamiel dose affected (P <.05) CO2 production (mL/g incubated DM) and proportional CO2 production at the incubated hours. Forage type aguamiel dose interactions were observed (P < .05) for CO2 production (mL/g incubated DM) and proportional CO2 production at the incubated hours but had no impact on CH4 production. It is concluded that addition of aguamiel to five forage species affected fermentation kinetics of gas production resulting in different in vitro cecal gas, CH4 and CO2 productions from these substrates.

Article

research subject categories CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Lipofection improves gene targeting efficiency in E14 TG2a mouse embryonic stem cells

SANDRA MARICRUZ LOPEZ HEYDECK Marcos Cajero Juárez ROGELIO ALEJANDRO ALONSO MORALES JOSE SIMON MARTINEZ CASTAÑEDA Jose Francisco Robles González ALBERTO BARBABOSA PLIEGO JUAN CARLOS VAZQUEZ CHAGOYAN (2009)

Electroporation has been the method of election for transfection of murine embryonic stem cells for over 15 years; however, it is a time consuming protocol because it requires large amounts of DNA and cells, as well as expensive and delicate equipment. Lipofection is a transfection method that requires lower amounts of cells and DNA than electroporation, and has proven to be effi cient in a large number of cell lines. It has been shown that after lipofection, mouse embryonic stem cells remain pluripotent, capable of forming germ line chimeras and can be transfected with greater effi ciency than with electroporation; however, gene targeting of mouse embryonic stem cells by lipofection has not been reported. The objective of this work was to fi nd out if lipofection can be used as effi ciently as electroporation for regular gene targeting protocols. This context compares gene targeting effi ciency between these techniques in mouse embryonic stem cells E14TG2a, using a gene replacement type vector. No differences were found in gene targeting effi ciency between groups; however, lipofection was three times more effi cient than electroporation in transfection effi ciency, which makes lipofection a less expensive alternative method to produce gene targeting in mouse embryonic stem cells.

Article

veterinaria gene targeting homologous recombination mouse embryonic stem cells transfection electroporation lipofection CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Fecal Gas Production of Ten Common Horse Feeds Supplemented With Saccharomyces cerevisiae

MONA MOHAMED MOHAMED YASSEEN ELGHANDOUR MIguel Mellado Ahmed E. Kholif Abdelfattah Zeidan Mohamed Salem ALBERTO BARBABOSA PLIEGO SUSANA BALLINAS ARCHUNDIA JOSE ALEJANDRO ESQUIVEL SALAZAR Nicholas Odongo (2016)

Concentrate feeds are needed when a horse cannot meet its energy and protein requirements from forage alone. Straws and hays are the most popular and less expensive sources of fiber for horses. Moreover, forage feeding to horses can provide many of the essential nutrients and prevent nutritional disorders because forage fibers maintain gastrointestinal health and well-being of horses [2]. Increasing dietary fiber to at least 1% of the horse’s body weight with decreasing starch and sugar levels can reduce such disorders [2]. Therefore, feeding adequate amounts of fibrous feeds is required for normal digestive system function.

The study aimed to assess the nutritive value of 10 feeds (grains and forages) commonly used in horse nutrition in Mexico, on the basis of their chemical composition, in vitro organic matter digestibility (IVOMD) and in vitro gas production measurements with or without the supplementation of Saccharomyces cerevisiae (SC) at 4 mg/g DM. Fecal inoculum was obtained from 4 adult English Thoroughbred horses fed on restricted amount of concentrate and oat hay ad libitum. Substrates tested were: 6 concentrates (corn gluten meal, soybean meal, steam-rolled corn, steam-rolled barley, oat grain, and wheat bran) and 4 roughages (soybean hulls, corn stover, alfalfa hay, and oat hay). Gas production (GP) was recorded at 2, 4, 6, 8, 10, 12, 14, 24, 48, and 70 hours using the pressure transducer technique. Some ingredient yeast interactions were observed (P .020) for the asymptotic GP and GP at 48 and 70 hours of incubation. Yeast addition increased (P <.001) the asymptotic GP of concentrates compared to roughages. Concentrate feeds had higher (P <.05) GP and lower (P <.001) rate of GP compared to roughages without yeast. From 24 to 70 hours of incubation, forages with or without yeast had lower (P < .05) GP compared to concentrates supplemented with SC. Forages had higher fermentation pH compared to concentrates but lower (P < .05) metabolizable energy, IVOMD, and microbial protein production compared to concentrates. Supplementation with SC increased (P < .05) the asymptotic GP of oat grain, soybean meal, soybean meal, steam-rolled barley, steam-rolled corn, wheat bran, corn stover, and oat hay, without affecting the rate of GP or lag time of oat grain, soybean meal, wheat bran, corn stover, and oat hay. Moreover, supplementation with SC increased (P < .05) metabolizable energy, IVOMD, and microbial protein production of steam-rolled barley, wheat bran, and corn stover, without affecting (P > .05) the fermentation of other feeds. Supplementation with SC improved fermentation of feeds with higher effects on concentrates compared to roughages. It was concluded that although SC mainly improves concentrate utilization by horses, it also improves fiber digestion when used on high-roughage diets fed to horses.

Article

Yeast Nutritive value Gas production Feeds Fecal inoculum BIOLOGÍA Y QUÍMICA

Antimicrobial and bactericidal impacts of Bacillus amyloliquefaciens CECT 5940 on fecal shedding of pathogenic bacteria in dairy calves and adult dogs

PAULINA VAZQUEZ MENDOZA MONA MOHAMED MOHAMED YASSEEN ELGHANDOUR Peter Adeniyi PEDRO SANCHEZ APARICIO MARIA UXUA ALONSO FRESAN ALBERTO BARBABOSA PLIEGO Abdelfattah Zeidan Mohamed Salem (2018)

The administration of B. amyloliquefaciens CECT 5940 to the dog diet has no significant effect on the hardness of the stool. Meanwhile, the bacillus count increases while the coliforms count decreases upon B. amyloliquefaciens CECT 5940 administration. This reveals that B. amyloliquefaciens CECT 5940 survived the gastrointestinal passage and rapidly colonized the dog intestine, which could positively affect the metabolism and composition of the intestinal microflora. These results show that B. amyloliquefaciens are a promising probiotic with an antimicrobial and bactericidal activities against the intestinal pathogenic bacteria for dairy calves and adult dogs.

Two experiments were carried out to evaluate the bactericidal impacts of Bacillus amyloliquefaciens CECT 5940 on the shedding of faecal pathogenic bacteria in dairy calves (Experiment 1) and in adults dogs (experiment 2). In the calves experiment, a completely randomized design was used to investigate the faecal bacteria profile of Holstein dairy calves fed with either pasteurized waste milk (PWM; n = 9) or a formulated non-medicated milk replacer (NMR; n = 9) for 60 d. The NMR containing sodium-butyrate and the active probiotic B. amyloliquefaciens CECT 5940. In the dogs experiment, addition of same probiotic (i.e., B. amyloliquefaciens CECT 5940) was carried out in two stages. The first stage started from day 7–37, and the second from day 44–71. The assessment of faecal score measured on day 22, 37, 42, 57, 71 and 77 to determine the texture of the stools. Calves received PWM consumed (P < 0.05) more starter feed between day 16 and day 45. The calves fed NMR had more moisture faeces and less cough reflux than the PWM-calves. Feeding NMR to calves increased faecal Klebsiella oxytoca and Proteus vulgaris counts in comparison to PWM-calves.

Article

Bacillus amyloliquefaciens Calf Dog Faecal bacteria Waste milk MEDICINA Y CIENCIAS DE LA SALUD

In Vitro Cecal Gas and Methane Production of Soybean Hulls–Containing Diets in the Presence of Salix babylonica Extract as a Fermentation Modulator in Horses

MONA MOHAMED MOHAMED YASSEEN ELGHANDOUR Jessica Chavez Cardenas-Chantres ALEJANDRO ESQUIVEL VELAZQUEZ ALBERTO BARBABOSA PLIEGO MOISES CIPRIANO SALAZAR Abdelfattah Zeidan Mohamed Salem (2017)

The aim of the present study was to evaluate the cecal gas production (GP) and methane (CH4) production as well as cecal fermentation kinetics when corn grain (CG) was replaced with soybean hulls (SHs) in horse diets in the presence of different levels of Salix babylonica (SB) extract. Corn grains were replaced with SH at different levels (/kg): 0 g (control), 75 g (SH75), or 150 g (SH150), with the inclusion of SB extract at: 0, 0.6, 1.2, and 1.8 mL/g dry matter (DM) of substrates. Ration type extract dose interactions were observed for GP and CH4 production at some incubation hours. Diets containing SH, without the inclusion of SB extract, increased the asymptotic GP (P ¼ .031) and decreased (P <.01) the rate of GP and lag time of GP. The inclusion of SB increased (P ¼.009) the rate of GP, without affecting the asymptotic GP or lag time of GP. Besides, the SH-containing rations decreased (P < .05) CH4 production, with no effect for SB extract dose. The SH75 ration increased (P < .05) cecal fermentation pH, metabolizable energy, short chain fatty acids, and gas yield at 24 hours of incubation, but quadratically decreased partitioning factor at 24 hours of incubation (P ¼ .023), whereas SB extract dose had no effect. It is concluded that SH-containing rations had higher potential fermentation efficiency and fermentation kinetics superior to that of CG. The level of 75 g SH/kg DM was the best level of inclusion to replace 30% CG in the diets of horses. The inclusion of SB extract did not affect the cecal fermentation kinetics of horse diets containing SH at different levels.

Article

Cecal fermentation Corn grain Horse nutrition Salix babylonica extract Soybean hulls BIOLOGÍA Y QUÍMICA

Digestion, growth performance and caecal fermentation in growing rabbits fed diets containing foliage of browse trees

SALMA H. ABU HAFSA Abdelfattah Zeidan Mohamed Salem ALAIN HASSAN ALTAMIRANO ESPINOZA Ahmed E. Kholif MONA MOHAMED MOHAMED YASSEEN ELGHANDOUR ALBERTO BARBABOSA PLIEGO SECUNDINO LOPEZ PUENTE (2016)

Feeding foliage from browse trees and shrubs may be of importance in animal production because these resources do not compete with human food and can provide significant amounts of nutrients. Rabbit is increasingly becoming an important meat source and its production is recommended in countries that are experiencing meat shortages, as it presents the best productive advantages to bridge the protein deficiency gap (Abdel-Aziz et al., 2015).

This study aimed to evaluate the effect of feeding dried foliage (leaves and petioles) of Acacia saligna, Leucaena leucocephala or Moringa oleifera on the performance, digestibility, N utilisation, caecal fermentation and microbial profiles in New Zealand White (NZW) rabbits. One hundred weaned male NZW rabbits weighing 819.2±16.6 g and aged 35±1 d were randomly allocated into 4 groups of 25 rabbits each. Rabbits were fed on pelleted diets containing 70% concentrate mixture and 30% Egyptian berseem (Trifolium alexandrinum) hay (Control diet) or one of the other 3 experimental diets, where 50% of berseem hay was replaced with A. saligna (AS), L. leucocephala (LL) or M. oleifera (MO). Compared to Control diet, decreases in dry matter (DM; P=0.004), organic matter (P=0.028), crude protein (CP; P=0.001), neutral detergent fibre (P=0.033) and acid detergent fibre (P=0.011) digestibility were observed with the AS diet. However, DM and CP digestibility were increased by 3% with the MO diet, and N utilisation was decreased (P<0.05) with AS. Rabbits fed AS and LL diets showed decreased (P=0.001) average daily gain by 39 and 7%, respectively vs. Control. Feed conversion was similar in Control and MO rabbits, whereas rabbits fed AS diet ate up to 45% more feed (P=0.002) than Control rabbits to gain one kg of body weight. Caecal ammonia-N was increased (P=0.002) with LL, while acetic acid was decreased (P=0.001) with AS diet vs. other treatments. Caecal E. coli and Lactobacillus spp. bacteria counts were decreased with MO by about 44 and 51%, respectively, vs. Control. In conclusion, under the study conditions, tree foliage from M. oleifera and L. leucocephala are suitable fibrous ingredients to be included up to 150 g/kg in the diets of growing rabbits, and can safely replace 50% of berseem hay in diets of NZW rabbits without any adverse effect on their growth performance. Foliage from M. oleifera had a better potential as a feed for rabbits than that from L. leucocephala. Although foliage from A. saliga may be also used at 150 g/kg in the diets of growing rabbits, this level of inclusion may result in reduced feed digestibility and growth performance.

Article

Caecal fermentation Digestion Rabbits Tree foliage BIOLOGÍA Y QUÍMICA