Author: ALBERTO CORTES VELAZQUEZ

First report of papaya meleira virus (PMeV) in Mexico

DAISY DE LA CARIDAD PEREZ BRITO RAUL TAPIA TUSSELL ALBERTO CORTES VELAZQUEZ ANDRES FELIPE DE JESUS QUIJANO RAMAYO ANGEL NEXTICAPAN GARCEZ RODOLFO MARTIN MEX (2012)

Papaya meleira virus (PMeV), causal agent of meleira or sticky disease, is a double-stranded RNA (dsRNA) virus which has been previously reported only in Brazil. A study was carried out in order to verify the presence and occurrence of PMeV in Mexico. Latex samples from symptomatic and asymptomatic papaya fruits were collected in Quintana Roo state papaya orchards, where the first symptoms of PMeV were observed, and from 29 different municipalities located in ten papaya producer states in Mexico. A molecular protocol based on nucleic acid extraction was used for the diagnosis and a virus 12 Kb dsRNA distinctive band was observed in all PMeV infected plants. Around 46% of the evaluated plants were positive for this pathogen. Presence of the virus had been confirmed in seven states indicating the potential damage that PMeV could cause in the papaya crop in Mexico. The molecular analysis used allowed the diagnosis of infected plants without symptoms and facilitated the diagnosis in flowers and small papaya fruits also. The early diagnosis of PMeV will allow papaya producers to take appropriate and timely control measures. This is the first report of Papaya meleira virus in Mexico.

Article

PAPAYA MELEIRA VIRUS STICKY DISEASE DSRNA NUCLEIC ACID ANALYSIS BIOLOGÍA Y QUÍMICA

Laccase gene expression and vinasse biodegradation by Trametes hirsuta strain Bm-2

RAUL TAPIA TUSSELL DAISY DE LA CARIDAD PEREZ BRITO CLAUDIA GUADALUPE TORRES CALZADA ALBERTO CORTES VELAZQUEZ LILIANA MARIA ALZATE GAVIRIA RUBI DEL ROSARIO CHABLE VILLACIS SARA ELENA SOLIS PEREIRA (2015)

Vinasse is the dark-colored wastewater that is generated by bioethanol distilleries from feedstock molasses. The vinasse that is generated from molasses contains high amounts of pollutants, including phenolic compounds and melanoindin. The goal of this work was to study the expression of laccase genes in the Trametes hirsuta strain Bm-2, isolated in Yucatan, Mexico, in the presence of phenolic compounds, as well as its effectiveness in removing colorants from vinasse. In the presence of all phenolic compounds tested (guaiacol, ferulic acid, and vanillic acid), increased levels of laccase-encoding mRNA were observed. Transcript levels in the presence of guaiacol were 40 times higher than those in the control. The lcc1 and lcc2 genes of T. hirsuta were differentially expressed; guaiacol and vanillin induced the expression of both genes, whereas ferulic acid only induced the expression of lcc2. The discoloration of vinasse was concomitant with the increase in laccase activity. The highest value of enzyme activity (2543.7 U/mL) was obtained in 10% (v/v) vinasse, which corresponded to a 69.2% increase in discoloration. This study demonstrates the potential of the Bm-2 strain of T. hirsuta for the biodegradation of vinasse.

Article

TRAMETES HIRSUTA LACASSES GENE EXPRESSION BIODEGRADATION VINASSE BIOLOGÍA Y QUÍMICA BIOLOGÍA Y QUÍMICA

Laccase gene expression and vinasse biodegradation by Trametes hirsuta strain Bm-2

RAUL TAPIA TUSSELL DAISY DE LA CARIDAD PEREZ BRITO CLAUDIA GUADALUPE TORRES CALZADA ALBERTO CORTES VELAZQUEZ LILIANA MARIA ALZATE GAVIRIA RUBI DEL ROSARIO CHABLE VILLACIS SARA ELENA SOLIS PEREIRA (2015)

Vinasse is the dark-colored wastewater that is generated by bioethanol distilleries from feedstock molasses. The vinasse that is generated from molasses contains high amounts of pollutants, including phenolic compounds and melanoindin. The goal of this work was to study the expression of laccase genes in the Trametes hirsuta strain Bm-2, isolated in Yucatan, Mexico, in the presence of phenolic compounds, as well as its effectiveness in removing colorants from vinasse. In the presence of all phenolic compounds tested (guaiacol, ferulic acid, and vanillic acid), increased levels of laccase-encoding mRNA were observed. Transcript levels in the presence of guaiacol were 40 times higher than those in the control. The lcc1 and lcc2 genes of T. hirsuta were differentially expressed; guaiacol and vanillin induced the expression of both genes, whereas ferulic acid only induced the expression of lcc2. The discoloration of vinasse was concomitant with the increase in laccase activity. The highest value of enzyme activity (2543.7 U/mL) was obtained in 10% (v/v) vinasse, which corresponded to a 69.2% increase in discoloration. This study demonstrates the potential of the Bm-2 strain of T. hirsuta for the biodegradation of vinasse.

Article

TRAMETES HIRSUTA LACASSES GENE EXPRESSION BIODEGRADATION VINASSE BIOLOGÍA Y QUÍMICA

Molecular characterization of Yucatan tomato phytoplasma (Group 16Sr III)

RAUL TAPIA TUSSELL ALBA PRISCILIA SUASTE DZUL ALBERTO CORTES VELAZQUEZ CLAUDIA GUADALUPE TORRES CALZADA ANDRES FELIPE DE JESUS QUIJANO RAMAYO RODOLFO MARTIN MEX ANGEL NEXTICAPAN GARCEZ DAISY DE LA CARIDAD PEREZ BRITO (2012)

Tomato (Lycopersicon esculentum) is an important vegetable crop in Mexico. Recently, a phytoplasma associated with leaf yellowing and curling, severe stunting and little leaf in tomato plant was identified as Yucatan tomato phytoplasma (16SrIII group). DNAs extracted from tomato leaves with symptoms were examined for the presence of this phytoplasma by nested polymerase chain reaction (PCR). Positive results were obtained in 44% of samples, yielding an rDNA product of 1.25 kb. In vitro and in silico restriction fragment length polymorphism (RFLP) patterns obtained with endonucleases HpaII, MseI, RsaI and TaqI were characteristics of group 16SrIII, according to the classification scheme of phytoplasmas. The pattern with AluI and HaeIII discriminated between these phytoplasmas and the members of 16SrIII group. Molecular characterization of the causal agent of Yucatan tomato phytoplasma will facilitate the study of this disease’s epidemic aspects and its phytosanitary management. In addition, it will contribute to a greater knowledge of the genetic diversity of phytoplasmas present in Mexico.

Article

DIAGNOSTICS PHYTOPLASMA 16S RDNA PCR-RFLP BIOLOGÍA Y QUÍMICA

Zoonosis, cambio climático y sociedad

CAMILO ROMERO NUÑEZ Ranulfo Pérez Garcés JUAN JOSE OJEDA CARRASCO ROGER IVAN RODRIGUEZ VIVAS MANUEL EMILIO BOLIO GONZALEZ MELINA MARIBEL OJEDA CHI José Alberto Rosado Aguilar IRIS DEL CARMEN TRINIDAD MARTINEZ EDWIN JOSE GUTIERREZ RUIZ Enrique Alberto Reyes Novelo Juan Pablo Martínez Labat ELIZABETH ORTEGA SOTO BLANCA LILIA BARRON ROMERO RAFAEL HEREDIA CARDENAS LUCILA MARILU RODRIGUEZ GALLEGOS NADYELI NAVA CORTES ALEJANDRO DE LA PEÑA MOCTEZUMA HECTOR MANELIC QUIROZ ROMERO GERMAN DAVID MENDOZA MARTINEZ Angélica Hernández Estrada LINDA GUILIANA BAUTISTA GOMEZ Hugo Barajas Rubio VIRGINIA GUADALUPE GARCIA RUBIO CARLOS RAMON BAUTISTA GARFIAS Paola Edith Nava León TANIA OLIVIA ROJAS CAMPOS JOCELYN PINEDA CRUZ MINERVA ARCE FONSECA Rodrigo Alexis Arias Inostroza VALENTE VELAZQUEZ ORDOÑEZ BENJAMIN VALLADARES CARRANZA MARIA UXUA ALONSO FRESAN JORGE PABLO ACOSTA DIBARRAT (2015)

La sociedad contemporánea se enfrenta a uno de los retos más grandes de la historia humana, el calentamiento global, mismo que acarrea enormes consecuencias, tales como los disturbios climáticos, así como los patrones de las enfermedades de origen animal transmisibles al hombre. Precisamente ante este escenario las instituciones educativas de nivel superior deben dar cumplimiento a su responsabilidad y ser las generadoras de alternativas de solución mediante el trabajo especializado de investigación; y para ello, la pesquisa científica es la mejor de las alternativas a nuestro alcance para comprender y encarar estos desafíos.

Universidad Autónoma del Estado de México y Ediciones y Gráficos Eón, S.A. de C.V.

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zoonosis cambio climático sociedad clima desarrollo seguridad alimentaria calentamiento global enfermedad animal CIENCIAS SOCIALES